Preparation method of multi-responsive self-healing self-adhesive hydrogel
A hydrogel and responsive technology, applied in the field of preparation of multi-responsive self-healing self-adhesive hydrogels, can solve the problems of lack of tissue adhesion and cell affinity, poor mechanical properties of hydrogels, etc. , to achieve the effect of wide application potential, simple preparation method and good biocompatibility
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Embodiment 1
[0025] At room temperature, take 36.9 g of bacterial cellulose suspension with a mass fraction of 8.13 mg / g (about 0.3 g of bacterial cellulose), disperse it into 30 mL of tris buffer with a pH value of 8.5, and then add 0.2 g Tannic acid, stir evenly, after the prepolymerization reaction for 6 hours, add 0.2 g of cystamine dihydrochloride to continue the reaction for 1.5 hours to obtain the bacterial cellulose suspension coated with tannic acid, and successfully introduce disulfide bonds, and the suspension Centrifuge and wash for later use.
[0026] Add 5g of polyvinyl alcohol into 45mL of deionized water and heat to 95°C, stir for 2 hours until the polyvinyl alcohol is completely dissolved, add the centrifuge obtained in the previous step into the polyvinyl alcohol solution and continue to stir evenly, then add 5mL of the concentration to 0.04g / Add 1 mL of borax solution dropwise at a rate of 1 mL / min, stir for 20 min, and then naturally cool to room temperature to obtain ...
Embodiment 2
[0030] At room temperature, take 36.9 g of bacterial cellulose suspension with a mass fraction of 8.13 mg / g (about 0.3 g of bacterial cellulose), disperse it into 30 mL of tris buffer with a pH value of 8.5, and then add 0.5 g Tannic acid, stir evenly, after the prepolymerization reaction for 6 hours, add 0.2 g of cystamine dihydrochloride to continue the reaction for 1.5 hours to obtain the bacterial cellulose suspension coated with tannic acid, and successfully introduce disulfide bonds, and the suspension Centrifuge and wash for later use.
[0031] Add 5g of polyvinyl alcohol into 45mL of deionized water and heat to 95°C, stir for 2 hours until the polyvinyl alcohol is completely dissolved, add the centrifuge obtained in the previous step into the polyvinyl alcohol solution and continue to stir evenly, then add 5mL of the concentration to 0.04g / Add 1 mL of borax solution dropwise at a rate of 1 mL / min, stir for 20 min, and then naturally cool to room temperature to obtain ...
Embodiment 3
[0034] At room temperature, take 36.9 g of bacterial cellulose suspension with a mass fraction of 8.13 mg / g (approximately containing 0.3 g of bacterial cellulose), disperse it into 30 mL of tris buffer with a pH value of 8.5, and then add 0.8 g Tannic acid, stir evenly, after the prepolymerization reaction for 6 hours, add 0.2 g of cystamine dihydrochloride to continue the reaction for 1.5 hours to obtain the bacterial cellulose suspension coated with tannic acid, and successfully introduce disulfide bonds, and the suspension Centrifuge and wash for later use.
[0035] Add 5g of polyvinyl alcohol into 45mL of deionized water and heat to 95°C, stir for 2 hours until the polyvinyl alcohol is completely dissolved, add the centrifuge obtained in the previous step into the polyvinyl alcohol solution and continue to stir evenly, then add 5mL of the concentration to 0.04g / Add 1 mL of borax solution dropwise at a rate of 1 mL / min, stir for 20 min, and then naturally cool to room tem...
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