Method for separating and extracting casein glycomacropeptide
A technology for casein glycomacropeptide and casein, which is applied in the field of separation and extraction of casein glycomacropeptide, can solve the problems that the molecular weight range of the filter membrane is not easy to select accurately, the product contains less impurities and purity, and the equipment cost is high, and it is easy to popularize. , The effect of low equipment requirements and low impurity content
Active Publication Date: 2021-08-06
NEW HOPE DAIRY HLDG
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[0004] The purpose of the present invention is: for the existing method of using membrane ultrafiltration to intercept and separate glycomacropeptide in the prior art, the equipment cost requirement is high, the operation is complicated, and it is not easy to control. It is not easy to popularize, and it will also retain some impurities with similar molecular weight and the molecular weight range of the filter membrane is not easy to accurately select. A method for separating and extracting casein glycomacropeptide is provided. This method is simple to operate, low in equipment requirements, easy to control, and easy to promote. The product contains less impurities and has high purity
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Embodiment 1
[0043] Extraction and Separation of Casein Glycomacropeptide
[0044] Step 1. Take an appropriate amount of raw milk, centrifuge it at 8000r / min and 3°C for 10 minutes to remove fat, and take skim milk for later use.
[0045] Step 2. Take the skim milk and raise the temperature to 30°C. Under the condition of stirring, adjust the pH value to 4.7 with 1mol / L hydrochloric acid, collect the precipitate S1, wash the precipitate S1 with pure water, and set aside.
[0046] Step 3. Take the precipitate S1 and put it in pure water, adjust the pH value to 11 with 1mol / L sodium hydroxide, dissolve it under stirring at 45°C, and adjust the pH value to 6.86 at a low temperature of 3°C.
[0047] Step 4. Take the aqueous solution with a pH value of 6.86, add rennet (dissolved in 1 mol / L NaCl), enzymolyze it in a water bath at 35°C for 45 minutes, then add 0.5% trichloroacetic acid, and centrifuge to obtain supernatant L1.
[0048] Step 5. Take the supernatant L1 and add 8% trichloroacetic ...
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The invention relates to a method for separating and extracting casein glycomacropeptide. The method mainly comprises the following steps of: step 1, dissolving casein in an aqueous solution with a pH value of 5.0-6.86, then adding chymosin, and performing enzymolysis in a water bath to obtain a first material; step 2, mixing the first material obtained in the step 1 with a first trichloroacetic acid solution with a mass concentration of 0.4-0.6%, and centrifuging to obtain a supernatant; step 3, mixing the supernatant obtained in the step 2 with a second trichloroacetic acid solution with a mass concentration higher than 8%, centrifuging, and collecting a precipitate; and step 4, washing the precipitate obtained in the step 3 with acetone, and drying to obtain the casein glycomacropeptide. The extraction and separation method provided by the invention is simple to operate, low in equipment requirement, easy to control and convenient to popularize, and the product is low in impurity content and high in purity.
Description
technical field [0001] The invention relates to the technical field of separating and extracting glycomacropeptide, in particular to a method for separating and extracting casein glycomacropeptide. Background technique [0002] The protein in milk is mainly composed of whey protein and casein, among which casein is composed of α-casein, β-casein and κ-casein, and κ-casein has glycosylation structure, and glycosylated Glycomacropeptide is produced from κ-casein after enzymatic hydrolysis. Glycomacropeptide has the physiological activity of improving immunity. Glycomacropeptide has been applied in many fields. [0003] At present, the method for preparing casein glycomacropeptide is mainly enzymatic hydrolysis. For example, Chinese patent CN101413019A discloses a method for preparing casein glycomacropeptide. Then the supernatant was concentrated by membrane ultrafiltration to obtain casein glycomacropeptide. In addition, the Chinese patent CN103382497 discloses the preparat...
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Login to View More IPC IPC(8): C07K14/47C12P21/06C07K1/14
CPCC07K14/4732C12P21/06
Inventor 周欢谭莲英范光彩刘虎传夏忠悦宋艳梅钱成林骆敏马静
Owner NEW HOPE DAIRY HLDG