Primer for detecting panda-derived babesia as well as kit and detection method thereof

Abstract

The invention discloses a primer for detecting panda source babesia as well as a kit and a detection method thereof. The primers comprise outer primers as shown in SEQ ID NO. 1 and SEQ ID NO. 2, and inner primers as shown in SEQ ID NO. 3 and SEQ ID NO. 4. The detection method comprises the following steps: (1) extracting panda blood DNA for later use; (2) taking the DNA obtained in the step (1) as a template, and carrying out a first round of PCR by adopting an outer primer pair; and (3) carrying out a second round of PCR by taking the first round of PCR product as a template and adopting the inner primer pair, and then carrying out gel electrophoresis and judging whether the babesia is contained or not. Four specific primers are designed aiming at a specific region taking 18S rRNA of the panda-derived babesia as a target gene, so that the problem of lack of a molecular detection method aiming at the panda-derived babesia is solved, and a specific and sensitive early diagnosis method for the panda-derived babesia is realized.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a primer for detecting Babesia derived from giant pandas, a kit and a detection method thereof. Background technique [0002] Babesia is an apicomplexan protozoa that parasitizes in the erythrocytes of vertebrates. Ticks are the main transmission medium, and erythrocytes are the only host cells that can infect. Babesiosis caused by Babesia infection is a worldwide blood protozoan disease, and its main clinical symptoms are high fever, anemia, jaundice and hemoglobinuria. More than 100 species of Babesia have been identified so far. The disease not only causes huge losses to animal husbandry and the national economy, but also seriously endangers the health of wild animals and population protection. It can harm animals such as canines, cats, raccoons, deer and primates, and some species can also be infected by humans. Report. Babesia from giant pandas (GenBank: MT256...

AI Technical Summary

Problems solved by technology

Therefore, the lack of specific primers for Babesia from giant pandas significantly affects the detection rate of Babesia from giant pandas. At present, it is urgent to explore a specific primer and detection method for the detection of Babesia from giant pandas

[0003] For this disease, the most basic detection method is the blood smear microscopy method, which has a low detection rate and cannot distinguish the insect species, and cannot achieve early diagnosis. It is currently used as an auxiliary detection method in the acute onset stage; clinically Serological detection is often used for Babesia epidemiological research, but since there is no antibody against giant pandas on the market, the antibodies used in the detection have large species differences, which cannot accurately reflect the giant panda's infection with Babesia Therefore, in laboratory diagnosis, molecular diagnostic techniques are most commonly used, among which the common PCR detection technique is the most commonly used, but this method will have the problem of low sensitivity when detecting Babesia giant pandas , when the amount of worms carried by giant pandas is low, there will be problems such as missed detection, which is not conducive to the early diagnosis of Babesia from giant pandas

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