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Human small cell lung cancer cell strain with combined drug resistance to etoposide and carboplatin as well as establishment method and application thereof

A technology of small cell lung cancer and etoposide, which is applied in the field of human small cell lung cancer cell line and its establishment, can solve the problem of unclear molecular mechanism of carboplatin resistance, and achieve stable cell properties, wide application range and rich cell bank. Effect

Active Publication Date: 2021-08-10
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exact molecular mechanism of carboplatin resistance is still unclear

Method used

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  • Human small cell lung cancer cell strain with combined drug resistance to etoposide and carboplatin as well as establishment method and application thereof
  • Human small cell lung cancer cell strain with combined drug resistance to etoposide and carboplatin as well as establishment method and application thereof
  • Human small cell lung cancer cell strain with combined drug resistance to etoposide and carboplatin as well as establishment method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of human small cell lung cancer cell SCLC2018

[0048] NOD SCID mice, female, weighing 18-22g, aged 6-8 weeks, were raised in SPF environment. Mice were provided by Beijing Weitong Lihua Experimental Animal Co., Ltd.

[0049] (1) Obtain fresh clinical small cell lung cancer surgical resection samples from Hunan Cancer Hospital (male, 50 years old, small cell lung cancer tumor, clinical stage IV, resistant to the combination of etoposide and carboplatin, in line with ethics, and after The patient agrees), immediately put the tumor tissue into a sterile, 4°C pre-cooled tissue protection solution under aseptic conditions, transfer it to a pre-sterilized biological safety cabinet, take out the tissue from the centrifuge tube, and quickly transfer it to a 10cm and wash it twice with PBS containing double antibodies (100 U / mL penicillin and 100 μg / mL streptomycin), remove necrotic tissue and surrounding non-tumor tissue, and try to ensure that the necr...

Embodiment 2

[0056] The biological characteristic of embodiment 2 cells

[0057] The present invention uses RPMI 1640 medium to culture SCLC2018 cells, so that they can grow and pass stably in vitro for a long time. When the cells are passed on for more than 30 generations, the cell traits are gradually stabilized, and relevant biology, genetics, and tissue source identification are carried out until the 50th generation has the same stable traits. According to experimental observation and verification, SCLC2018 cells grown in vitro have a typical epithelial-like morphology, lose contact growth inhibition, and show malignant growth. The cells can form tumors in nude mice and are tumorigenic. This cell line can be used for research in vitro and anticancer drug sensitivity and drug resistance in vivo, providing new test materials for the occurrence, development and metastasis of human small cell lung cancer.

[0058] Morphological observation

[0059] Place the culture flask of cultured SCL...

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Abstract

The invention provides a human small cell lung cancer cell strain with combined drug resistance to etoposide and carboplatin as well as a preparation method and application thereof, wherein the human small cell lung cancer cell strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC No: C202168. The small cell lung cancer cell strain is stable in character, can be stably passed for multiple times, and can be used for generating human small cell lung cancer in mammals and preparing a human small cell lung cancer model; the cell is tolerant to combined medicine of etoposide and carboplatin which are first-line treatment medicines for small cell lung cancer, and can be used for researching a drug-resistant molecular mechanism of small cell lung cancer and candidating the drugs for treating drug-resistant human small cell lung cancer are screened. According to the invention, a new experimental material closer to clinical tumor biological characteristics is provided for small cell lung cancer research.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a human small cell lung cancer cell line with combined resistance to etoposide and carboplatin and its establishment method and application. Background technique [0002] Small cell lung cancer is a special pathological type of lung cancer, which is a highly malignant neuroendocrine tumor, accounting for 10% to 15% of the incidence of lung cancer. It is characterized by high degree of malignancy, rapid growth, short doubling time, strong invasiveness, and high susceptibility to metastasis. About 60% of patients have developed distant metastases at the time of treatment, and about 30% of them will develop bone metastases. Regional lymph nodes The median overall survival is only 8 months to 13 months, and the prognosis is poor. The five-year survival rate is less than 7%. Most patients die within 1 year after diagnosis. [0003] In the treatment of small cell lung cancer, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A01K67/027C12Q1/02A61K49/00
CPCC12N5/0693C12N5/0688A01K67/0271G01N33/5011A61K49/0008C12N2509/10C12N2509/00A01K2207/12A01K2227/105A01K2267/03C12N2503/02G01N2500/10
Inventor 杨农王建新许杰李敏张永昌
Owner CENT SOUTH UNIV
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