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Method for evaluating particulate chemicals released in consumer goods by simulating lung tissue liquid in-vitro substitution technology

A technology for consumer goods and particulate matter, applied in the field of exposure simulation, can solve problems such as overestimation of biological accessibility, difficulty in separation, and impact on the accuracy of results, to avoid overestimation of biological accessibility, low experimental cost, and convenient sample collection Effect

Inactive Publication Date: 2021-08-10
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, in this technical solution, due to the direct contact of particulate matter pollutants with the Tenax adsorbent, the particulate matter may enter the interior of the Tenax rod, making separation difficult, resulting in an overestimation of bioavailability and affecting the accuracy of the results.

Method used

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  • Method for evaluating particulate chemicals released in consumer goods by simulating lung tissue liquid in-vitro substitution technology
  • Method for evaluating particulate chemicals released in consumer goods by simulating lung tissue liquid in-vitro substitution technology
  • Method for evaluating particulate chemicals released in consumer goods by simulating lung tissue liquid in-vitro substitution technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for assessing particulate chemicals released from consumer products using an extrafluid replacement technique simulating lung tissue, comprising the following steps:

[0047] Preparation:

[0048] 1) Preparation of silica gel column: the chromatographic column is filled with glass fiber or empty SPE column and sieve plate, 1 g of acid silica gel (44% concentrated sulfuric acid), and 3 g of anhydrous sodium sulfate from bottom to top. The filled silica gel column was pre-cleaned with 6 mL of a mixture of n-hexane and dichloromethane (1:1 volume ratio of n-hexane and dichloromethane was 1:1).

[0049] The preparation method of acidic silica gel is as follows: dry and activate 100g of silica gel in an oven at 450°C for 6 hours, then take it out and put it in a drying oven to cool to room temperature; add 43mL of concentrated sulfuric acid to the silica gel drop by drop, and stir with a glass rod at the same time to prevent the silica gel from forming. block; afte...

Embodiment 2

[0075] A method of simulating the lung tissue liquid replacement technology to evaluate the release of particulate chemicals in consumer products. In this example, it is verified that the removal of the dialysis bag leads to the increase of the bioavailability of particulate matter into the Tenax structure, which is the same as that of Example 1. The difference is that instead of using a dialysis bag, the schoolbag particles and the Tenax device are directly placed in the simulated lung fluid, and the other steps are the same as those in Example 1.

[0076] In this example, the bioavailability fraction of SCCPs in schoolbag particulate matter was 15.85-35.65%. Among them, the bioavailability fraction of SCCPs in ALF solution was 15.85-26.48%; the bioavailability fraction of SCCPs in MGS solution was 17.36-35.65%. Compared to Example 1, SCCPs had an increased fraction of bioaccessibility due to the incorporation of particulate matter into the Tenax structure. Due to the need t...

Embodiment 3

[0078] A method for evaluating particulate chemicals released in consumer products by simulating lung tissue liquid replacement technology. In this example, the difference from Example 1 is that the particulate material is changed to an eraser, and the target pollutant is PAEs. Carry out solid-phase extraction with C18 glass column, the process internal standard is changed to D 4 -PAEs, other steps are similar to the method and steps of Example 1.

[0079] Three kinds of PAEs were detected in the lung fluid samples, namely DBP, DIBP, and DEHP, among which the bioaccessible fraction of DBP was 8.20-86.07%, the bioaccessible fraction of DIBP was 15.35-85.91%, and the bioaccessible fraction of DEHP 0.023-0.64%

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Abstract

The invention discloses a method for evaluating granular chemicals released in consumer goods by simulating a lung tissue liquid in-vitro substitution technology and relates to the technical field of consumer goods pollutant exposure simulation detection. According to the method, on the basis of simulated lung fluid, an in-vitro simulation device and a Tenax structure are used for carrying out an absorption test on pollutants in the simulated lung fluid, and a test result is beneficial for evaluating inhalation bioaccessibility of a pollutant particulate matter sample and is beneficial for analyzing the concentration level and composition characteristics of the pollutants migrated into the simulated lung fluid. The dialysis bag is used in the simulation process, the situation that the added particulate matter enters the Tenax structure and is difficult to separate from the Tenax structure, and consequently bioaccessibility is overestimated can be prevented, therefore, the bioavailability migration volume of the particulate target object released by the consumer goods through respiration can be evaluated more accurately, the evaluation result provides data for safety of pollutants, and a more scientific basis is provided for scientific limitation of pollutants in consumer goods and establishment of corresponding standards.

Description

technical field [0001] The invention relates to the field of exposure simulation technology, in particular to a method for simulating lung tissue liquid replacement technology to evaluate particulate chemicals released in consumer products. Background technique [0002] With the technological advancement of society, the number and types of consumer products used in daily life are increasing, some of which may contain different types of toxic and harmful compounds. During the daily use of consumer products, these compounds can be released into the environment in the form of gaseous and particulate forms, and can easily enter the human body through different channels such as the respiratory tract, oral cavity and skin, thereby posing a potential threat to human health. [0003] The air humans breathe contains particulate matter of various sizes and compositions, including mineral dust, metals, sea salt, nitrates, sulfates, elemental carbon, and organic and inorganic pollutants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00G01N33/00
CPCG01N15/00G01N33/00Y02A50/20
Inventor 李菊英王志方黄东航丁腾达
Owner SHENZHEN UNIV
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