A new interface for in vivo microdialysis mass spectrometry coupled with real-time online analysis technology
An analysis technology and microdialysis technology, applied in the field of new interfaces, can solve the problem that the combined technology cannot realize real-time online analysis and so on.
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Embodiment 1
[0037] The novel interface of the present invention for real-time on-line analysis technology of in vivo microdialysis mass spectrometry includes an ion source and a three-dimensional mobile platform. like figure 1 As shown, it is a structural schematic diagram of the ion source in the new interface of the present invention for in vivo microdialysis mass spectrometry coupled with real-time on-line analysis technology.
[0038] The ion source is a triple casing composed of an inner capillary 1, an intermediate capillary 2 and an outer stainless steel tube 3 that are sequentially sleeved from the inside to the outside; the inner capillary 1 is connected to the microdialysis probe, and the dialysate flows into the innermost capillary 1 . The voltage-applied organic solvent enters the interlayer capillary 2 through the organic solvent inlet 4 . Nitrogen enters the stainless steel tube 3 through the nitrogen inlet 5.
[0039] The sample and the organic solvent are mixed at the s...
Embodiment 2
[0042] Using the device for in vivo microdialysis mass spectrometry coupled with real-time online analysis technology in Example 1 of the present invention to perform online mass spectrometry analysis, the online extraction sample is used to separate from the salt, and the specific steps are as follows:
[0043] The stainless steel target plate is placed on a conventional horizontal experimental table, and the ion source is adjusted vertically downward through the three-dimensional mobile platform, perpendicular to the stainless steel target plate.
[0044] Physiological saline (0.9% NaCl) was injected into the innermost capillary 1 through the microdialysis probe at a flow rate of 2 μl / min through a syringe pump, and fluorescein isothiocyanate (FITC) dissolved in methanol at a concentration of 10 μM The organic solvent inlet 4 enters the middle layer capillary 2 at a flow rate of 10 μl / min. The nitrogen gas of 0.1 MPa enters the outermost layer pipe 3 through the nitrogen inle...
Embodiment 3
[0050] This example provides a long-term detection of the glucose standard (0.1mg / mL) dissolved in normal saline to verify the signal stability of the long-term detection of the new interface used in in vivo microdialysis mass spectrometry coupled with real-time on-line analysis technology in Example 1 of the present invention sex.
[0051] Glucose standard (0.1 mg / mL) dissolved in physiological saline was injected into the innermost capillary 1 at a flow rate of 2 microliters / min through a syringe pump. Apply 3kV positively charged methanol containing 0.1% formic acid to enter the middle layer capillary 2 through the organic solvent inlet 4 at a flow rate of 10 microliters / minute. The nitrogen gas of 0.1MPa enters the outer layer stainless steel tube 3 through the nitrogen inlet 5.
[0052] The mass spectrometer model used was Qtrap 4500 (Sciex, Foster, CA, USA).
[0053] The detection time is 90 minutes, such as Figure 4 As shown, the glucose signal remained stable throug...
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Abstract
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