Chrysanthemum chloroplast genomic SSR marker library, acquisition method and application thereof

Abstract

The invention discloses a chrysanthemum chloroplast genomic SSR (simple sequence repeat) marker library, an acquisition method and application thereof. The SSR marker library comprises an SSR marker sequence SEQ ID NO.1, and further comprises at least one of SEQ ID NO.2-40; the acquisition method of the SSR marker library comprises the following steps: performing SSR analysis on chloroplast genomic DNA of chrysanthemum varieties to obtain SSR markers, and splicing the SSR markers of the same variety into SSR marker sequences to obtain the SSR marker library; the application of the SSR marker library is characterized in that the SSR marker library is used for constructing a phylogenetic tree or identifying the chrysanthemum varieties; and the SSR marker library can be used for effectively and accurately identifying chrysanthemum varieties and carrying out chrysanthemum genetics research and breeding parent selection.

Description

technical field [0001] The invention relates to a chrysanthemum chloroplast genome SSR marker library, its obtaining method and application, and belongs to the technical field of molecular biology. Background technique [0002] Chrysanthemum plays a leading role in the exploration of flower resources, and plays a huge role in ornamental, edible and medicinal production. For chrysanthemum varieties and classification, there are related studies in the fields of traditional morphology, quantitative taxonomy, cytology, cluster analysis, etc., which provide a reference for us to understand the differences in chrysanthemum taxonomy and the genetic relationship of varieties, but these studies only focus on a small number of chrysanthemum species. Experimental analysis of chrysanthemum varieties cannot help us to identify chrysanthemums correctly and effectively. Although the identification of chrysanthemums by phenotype has the advantages of convenience and directness, the genetic...

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Problems solved by technology

For chrysanthemum varieties and classification, there are related studies in the fields of traditional morphology, quantitative taxonomy, cytology, cluster analysis, etc., which provide a reference for us to understand the differences in chrysanthemum taxonomy and the genetic relationship of varieties, but these studies only focus on a small number of chrysanthemum species. Experimental analysis of chrysanthemum varieties cannot help us to identify chrysanthemums correctly and effectively.

Although the identification of chrysanthemums by phenotype has the advantages of convenience and directness, the genetic situation becomes very complicated due to the variety of chrysanthemum varieties, small differences between varieties, parallel evolution of morphological characters, developmental variation subject to natural selection, and long-term hybridization. , leading to low identification efficiency and hindering plant reproduction and international exchanges. Therefore, there has been controversy in the classification of chrysanthemums, and it is impossible to quickly and effectively identify chrysanthemum varieties solely by morphological characteristics, cytology and other methods

[0003] At present, some scholars have developed and used molecular marker technology to study chrysanthemum at different levels, but the calculation process of these studies is complicated, suitable for small number of samples, insufficient primers or few research sites, and cannot effectively, correctly and quickly identify and distinguish most chrysanthemum species

Therefore, it is still very difficult to perfect a comprehensive and unified classification and identification method, and there are deficiencies and limitations in the classification of varieties and the study of genetic relationships.

First, the cultivation and breeding of chrysanthemums mostly start from the phenotype. Due to the similarity of the phenotypes of various varieties, it affects the acceptance of genetic traits, resulting in low efficiency of breeding new varieties

Second, there is assimilation of chrysanthemum phenotypes, small differences between varieties, and low identification accuracy, which seriously confuses chrysanthemum varieties and hinders communication and cultivation between varieties

The third is that the self-incompatibility and inbreeding of cultivated chrysanthemums lead to heterozygosity, which in turn delays genetic improvement

Fourth, the chrysanthemum classification and identification system is not perfect, which affects the tracing of its genetic relationship and origin, and the chrysanthemum is easy to hybridize to produce new variations, making its development system more complicated

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