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Immunomodulating compositions from bile

An immunomodulatory agent and composition technology, which is applied in the directions of drug combination, hydrocarbon compound active ingredient, hydroxyl compound active ingredient, etc., can solve the problem of bile not considering tumors or infectious diseases, etc.

Inactive Publication Date: 2003-12-31
爱林米尔生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bile has never been considered as a useful composition for the treatment of tumors or infectious diseases

Method used

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  • Immunomodulating compositions from bile
  • Immunomodulating compositions from bile
  • Immunomodulating compositions from bile

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0223] The preparation of embodiment 1 composition of the present invention

[0224] Bile is collected from healthy cattle at least one and a half years of age slaughtered for food by a licensed and inspected slaughterhouse. Gallbladders were collected from self-examined slaughtered animals, the gallbladders were separated from the livers and veterinary examination confirmed that the gallbladders were free of parasites and showed no signs of infection, therefore the gallbladders were suitable for use as a source of bile.

[0225] The gallbladder that passed the test was wiped clean with 70% ethanol, and the bile was drawn out with a syringe. The withdrawn bile in the syringe was visually inspected by a veterinarian to ensure that it contained no blood or pus and was otherwise satisfactory. Transfer a satisfactory amount of bile into a graduated amber bottle of ethanol. Bile is a green liquid that is mostly free of blood and pus. Add bile up to twice th...

Embodiment 2

[0236] Example 2 Physicochemical and Biochemical Properties of Compositions of the Invention

[0237] A number of physicochemical properties (conductivity, osmotic pressure and total solids) of the preparations are shown in Table 2. More particularly, the results of the examination of three production batches of the composition prepared according to the method of Example 1 were made. The results shown in Table 2 demonstrate the sterility, potency and reproducibility of the products produced. Please note that ethanol and ether are only measured in the test. Example 4 provides a summary of methods for assaying biological activity.

[0238] Table 2: Product Specification Inspection Specification Method Biological activity 18+ / -5 units / ml Biological activity identity / purity Consistent with the reference HPLC Safety Passed inspection General safety inspection

[0239] (mice and guinea pigs)

...

Embodiment 3

[0259] The biological activity of embodiment 3 composition components

[0260] The biological activity of the composition fractions was studied. The biological activity of the composition is believed to be due to small molecular weight components (molecular weight less than 3000 Da). This conclusion was confirmed experimentally by testing the bioactivity of the four composition fractions and the unfractionated composition. The first fraction contains proteins and peptides with a molecular weight less than 3000 Da, while the remaining three fractions contain proteins and peptides with an additional larger molecular weight. All fractions contained additional larger molecular weight proteins and polypeptides. All fractions and unfractionated compositions showed the same biological activity. Since all fractions are as potent as the unfractionated product, and since a common feature of all fractions is the presence of molecules less than 3000 Da in the same...

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PUM

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Abstract

The present invention relates to a composition for use as an immunomodulator comprising small molecular weight components of less than 3000 daltons, and having the following properties: a) is extractable from bile of animals; b) is capable of stimulating monocytes and macrophages in vitro; c) is capable of modulating tumor necrosis factor production; d) contains no measurable IL-1a, IL-1b, TNF, IL-6, IL-8, IL-4, GM-CSF or IFN-gamma; e) has an anti-proliferative effect in a malignant mouse hybridoma cell line; f) shows no cytotoxicity to human peripheral blood mononuclear cells; and g) is not an endotoxin. The invention also relates to a method of preparing the composition and its use an immunomodulator.

Description

field of invention [0001] The present invention relates to immunomodulatory compositions, medicaments containing said compositions and the use of said compositions and medicaments in treating animals. Background of the invention [0002] Therapies related to the prevention and treatment of cancer and infectious diseases such as acquired immunodeficiency syndrome (AIDS) are constantly being developed. Some of these treatments attempt to harness the immune system therapeutically. One approach is based on the antigen-specific components of the immune system, namely antibodies and T cells. For example, the goal of related research has focused on the development of vaccines against foreign substances or against certain endogenous chemical messengers (such as interleukins) to suppress antibody responses. The second approach is based on the isolation, cloning, expression and production of polypeptides and proteins of non-antigen-specific parts of the immune system. For example, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/00A61K31/01A61K31/07A61K31/08A61K31/13A61K31/185A61K31/195A61K31/20A61K31/215A61K31/56A61K31/575A61K31/685A61K31/70A61K31/715A61K35/413A61K38/01A61K38/17A61K45/00A61K45/06A61P35/00A61P37/00C07K14/47
CPCA61K35/413A61K31/20A61K31/07A61K31/185A61K31/685A61K38/1709A61K45/06A61K31/08A61K31/215A61K38/01A61K31/575C07K14/4705A61K31/13A61K31/195A61K31/70A61K31/56A61P13/08A61P15/00A61P35/00A61P35/02A61P37/00A61P37/02A61K2300/00
Inventor R·兰格
Owner 爱林米尔生物有限公司
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