Human chorionic gonadotropin peptide aptamer as well as preparation method and application thereof

A chorionic gonadotropin and peptide adaptation technology, applied in the fields of peptides, instruments, analytical materials, etc., can solve the problems of poor reagent stability, complex instruments, harsh antibody storage conditions, etc., and achieve high binding rate and good specificity.

Inactive Publication Date: 2021-08-17
长沙信励致和科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The detection range of Roche’s electrochemiluminescence method is 0.100-10000mIU/mL, which cannot meet the detection of HCG concentration exceeding 10000mIU/mL in the 12th to 14th week of pregnancy, so the detection often requires a second detection after dilution
This method has complex instruments, poor reagent stability, and harsh storage conditions for antibodies. It must be operated by professional laboratory personnel. Cross-contamination, the above defects make the

Method used

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  • Human chorionic gonadotropin peptide aptamer as well as preparation method and application thereof
  • Human chorionic gonadotropin peptide aptamer as well as preparation method and application thereof
  • Human chorionic gonadotropin peptide aptamer as well as preparation method and application thereof

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Embodiment 1

[0062] Embodiment 1 Human chorionic gonadotropin peptide aptamer of the present invention and preparation method thereof

[0063] 1. Experimental instruments and materials

[0064] -80 ℃ ultra-low temperature refrigerator (Zhongke Meiling, Hefei); precision electronic analytical balance (Mettler Toledo, Germany); constant temperature oscillator (BOYN, Hangzhou); centrifuge (Michael, Hunan); vortex mixer (Lepter Science) instrument, Beijing); pipette gun (Eppendorf, Germany); electrophoresis apparatus (Bio-Rad, USA); membrane transfer apparatus (Bio-Rad, USA); magnetic stirrer (Lei Magnetic, Shanghai); , USA); gel imaging system (Bio-Rad, USA); ultrapure water instrument (Heal Force, Hong Kong); metal bath thermostat (Kylin-Bell, Haimen); HCG-α monoclonal antibody (Leading Bio, Shanghai); peptide aptamer (Copeptide, Shanghai); tris (2-carboxyethyl) phosphine (TCEP) (Aladdin, Shanghai); Loading Buffer (Thermo, U.S.); ECL chromogenic solution (Thermo, U.S.); Marker (Thermo, U.S...

Embodiment 2

[0073] Example 2 Performance verification of the human chorionic gonadotropin peptide aptamer of the present invention

[0074] 2.1 Basic properties of peptide aptamers

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Abstract

The invention relates to the technical field of peptide aptamers, in particular to a human chorionic gonadotropin peptide aptamer. The aptamer comprises any one of the following polypeptide sequences: P1, MHLMRMKPLLLT; P2, MHPRKMLQLMLN; P3, STRLRRRSRRQT; P4, PPLRINRHILTR; P5, MKLKPMRIMINP; and P6, MKSRMLPLNRRL. The invention also provides a preparation method of the human chorionic gonadotropin peptide aptamer. The human chorionic gonadotropin peptide aptamer provided by the invention is good in HCG binding specificity and high in binding rate, and can more accurately reflect the HCG condition of a sample when being applied to HCG detection. The application of the human chorionic gonadotropin peptide aptamer includes but is not limited to manufacturing of a detection reagent for detecting HCG, manufacturing of a biological detection sensor for detecting HCG and the like.

Description

technical field [0001] The invention relates to the technical field of peptide aptamers, in particular to a human chorionic gonadotropin peptide aptamer and its preparation method and application. Background technique [0002] Human chorionic gonadotropin (Human Chorionic Gonadotropin, HCG) is a glycoprotein hormone secreted by the trophoblast cells of the placental chorionic vesicles. Composition, the molecular weight is about 36.7kDa. Structurally, the α subunit is similar to many hormones such as thyroid-stimulating hormone and follicle-stimulating hormone, while the β subunit is unique to HCG, so the clinical detection of HCG mainly utilizes the specificity of the β subunit. [0003] HCG is produced by the placenta, as are trophoblastic tumors, germ cell tumors containing trophoblastic tissue, and some nontrophoblast tumors. The serum of pregnant women mainly contains intact molecule HCG, and its concentration will increase exponentially in the early pregnancy and is r...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/76
CPCC07K7/08G01N33/76G01N2333/59
Inventor 余鹏赵佳
Owner 长沙信励致和科技有限公司
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