Microfluidic immunization and nucleic acid detection chip

Abstract

The invention discloses a microfluidic immunization and nucleic acid detection chip which comprises an immunization array chip detection module, a nucleic acid lysis-isothermal amplification detection module and a waste liquid pool; the immunization array chip detection module comprises a to-be-detected sample solution inlet, an immunization cleaning solution inlet, a secondary antibody solution inlet, an immunization reaction and detection chamber and a bent fluid channel; the nucleic acid lysis-isothermal amplification detection module comprises a nucleic acid lysis solution inlet, a nucleic acid cleaning solution inlet, a nucleic acid eluent inlet, a nucleic acid lysis chamber, a nucleic acid amplification reaction and detection chamber and a first outlet; and a first micro-fluid valve is arranged on a first channel, and a second micro-fluid valve is arranged on a second channel. The invention provides a microfluidic immunization and nucleic acid detection chip, which integrates sample introduction, treatment and detection, integrates immunization detection and nucleic acid detection, achieves fast, small and accurate effects, and realizes real-time or batch detection in a molecular detection technology.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, and more specifically relates to a microfluidic immune and nucleic acid detection chip. Background technique [0002] With the rapid development of biological science and medical technology, molecular detection technology is widely used in the field of medical detection and diagnosis. [0003] At present, there are still problems in sample preservation, sample extraction and processing, detection efficiency, sensitivity and reliability in molecular detection and clinical application of immune and nucleic acid detection. The main limitations are: [0004] 1. After biochemical samples are collected, they should be stored at room temperature for a short period of time (generally no more than 4 hours), and they can only be stored below -70°C for a long time. There is a risk of denaturation and inactivation of samples if they are not processed in time; [0005] 2. Immunoassays are mainly...

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Problems solved by technology

[0004] 1. After biochemical samples are collected, they should be stored at room temperature for a short period of time (generally no more than 4 hours), and they can only be stored below -70°C for a long time. There is a risk of denaturation and inactivation of samples if they are not processed in time;

[0005] 2. Immunoassays are mainly evaluated by the specific combination of antigen and antibody, while nucleic acid detection is mainly evaluated by target gene amplification and signal collection. The two detection samples cannot be used simultaneously or repeatedly, which greatly reduces the detection efficiency;

[0006] 3. Immunity and nucleic acid detection, the two detection principles are completely different, there is a risk of cross-contamination during protein or nucleic acid extraction, thus affecting the detection results;

[0007] 4. Traditional immunoassay and nucleic acid detection need to be equipped with supporting reagents and consumables, and because it takes a certain amount of time for molecules to fully combine, traditional methods cannot achieve real-time or batch detection

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