Stenotrophomonas FT2 and application of fertilizer and fungicide in broad-spectrum biocontrol and growth promotion

A Stenotrophomonas, broad-spectrum technology, applied in the application field of Stenotrophomonas FT2 and fertilizers and bacterial agents in broad-spectrum biocontrol and growth promotion, can solve pesticide residues and endanger human health and ecological environment Safety, environmental pollution and other issues, to achieve the effect of increasing the number, promoting germination and plant growth, improving soil enzyme activity and available nutrient content

Active Publication Date: 2021-08-24
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and excessive use of chemical pesticides and fertilizers has caused a series of problems such as environmental pollution, soil compaction, and pesticide residues, which seriously threaten human health and ecological environment safety.

Method used

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  • Stenotrophomonas FT2 and application of fertilizer and fungicide in broad-spectrum biocontrol and growth promotion
  • Stenotrophomonas FT2 and application of fertilizer and fungicide in broad-spectrum biocontrol and growth promotion
  • Stenotrophomonas FT2 and application of fertilizer and fungicide in broad-spectrum biocontrol and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Isolation of Stenotrophomonas FT2

[0036] Select healthy and plump licorice seeds, soak the seeds with concentrated sulfuric acid solution with a mass percentage of 85% for 45 minutes, stir occasionally, rinse with sterile water for 3 times, and then use 0.1% hydrogen peroxide The solution was soaked for 10 minutes, rinsed with sterile water for 5 times, and finally soaked in sterile water for 3 hours, and then transferred to an ultra-clean workbench for surface disinfection of the seeds. Under sterile conditions, soak in 75% ethanol solution by volume for 5 minutes, soak in 1% hydrogen peroxide solution for 30 seconds, soak in 5% sodium hypochlorite solution for 10 seconds, and rinse with sterile water for 10 seconds. Finally, absorb the liquid on the surface of the seeds with sterile filter paper, put them into a mortar, add 10 mL of sterile water to grind. Take 200 μL of the suspension and evenly spread it on the NA solid medium, culture it in an artificial climate...

Embodiment 2

[0038] The identification of the bacterial strain that embodiment 1 obtains

[0039] Streak inoculation of the bacterial strain obtained in Example 1 on LB medium, culture at a constant temperature of 28°C for 2-7 days, and record the color, shape, transparency, and bulging degree of the surface edge of the colony. The physiological and biochemical characteristics of the strains obtained in Example 1 were measured with reference to the "Berger Bacteria Identification Handbook" and "Common Bacterial System Identification Handbook". The physiological and biochemical characteristics are shown in Table 2.

[0040] Table 2 Some physiological and biochemical characteristics of Stenotrophomonas FT2 strain

[0041] feature result feature result feature result biochemical test Carbon source utilization Nitrogen utilization MR test － D-Mannitol + urea － V-P test － D-galactose + Glutamine + citrate test + D-xylose － Gl...

Embodiment 3

[0049] Determination of growth-promoting properties of Stenotrophomonas FT2 strain

[0050] Ability to produce IAA (indole acetic acid)

[0051] Pick a single colony of Stenotrophomonas FT2 strain and inoculate it in NA liquid medium containing tryptophan 0.5g / L (beef extract 3g, peptone 10g, NaCl 5g, ddH 2 O 1L, pH 7.2~7.4), culture at 28°C, 160r / min shaking for 3d, then centrifuge at 9000r / min for 10min, take 2mL supernatant and 4mL Salkowski reagent (0.5mM FeCl 3 and 50mL H 2 SO 4 Mixing), the color turns red after standing in the dark at 28°C for 30 minutes ( Figure 5 In a), it shows that the Stenotrophomonas FT2 strain can produce IAA, and the OD is determined 530 , is 0.2522, while drawing the IAA standard curve as shown in Table 1 and Figure 4 indicated, the OD will be determined 530 Substituting the standard curve to calculate the IAA content can reach 8.732mg / L.

[0052] Table 1 IAA standard curve data

[0053] Concentration (mg / ) 0 5 10 15 20...

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Abstract

The invention relates to the field of microorganisms, in particular to stenotrophomonas FT2 and application of fertilizer and fungicide in broad-spectrum biocontrol and growth promotion. The invention provides a strain of stenotrophomonas sp. FT2 with broad-spectrum biocontrol and growth promoting effects, wherein the preservation number of the stenotrophomonas FT2 is CGMCC No.21379. The strain has an inhibition effect on a plurality of fungal diseases, has the advantages of no toxicity, harmlessness and no pollution, can promote the germination of plant seeds and the growth of plants, improves the enzymatic activity of plant rhizosphere soil and the content of available nutrients, and increases the number of soil bacteria.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of Stenotrophomonas FT2, fertilizers and bacterial agents in broad-spectrum biocontrol and growth promotion. Background technique [0002] The occurrence of plant diseases often destroys the growth of plants, which can directly lead to the reduction of their yield and quality, and is one of the main factors threatening agricultural production. There are many types of soil-borne diseases of medicinal plants caused by factors such as continuous cropping, poor soil conditions in cultivation sites, and increased drug resistance of pathogenic bacteria. In addition, there are few systematic studies on medicinal plant diseases, making the prevention and control of medicinal plant diseases an important issue. Currently the weakest link in agricultural production. At present, the prevention and control of plant diseases mainly depends on the selection of chemical pesticides ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/20A01P3/00A01P21/00C12R1/01
CPCC05F11/08A01N63/20
Inventor 张新慧王兰梦李小康郎多勇郑立昊
Owner NINGXIA MEDICAL UNIV
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