Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ARID1A gene targeted-knockout sgRNA, method for constructing ARID1A gene deletion cell strain, and application

A gene deletion and cell line technology, applied in the field of biomedicine, to achieve the effects of high gene editing efficiency, simple construction method and strong operability

Pending Publication Date: 2021-08-27
THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the design of ARID1A gene knockout sgRNA related to gastric cancer in China. Therefore, by designing ARID1A gene knockout sgRNA, we can construct a stable human gastric epithelial cell line with ARID1A mutation deletion that can be cultured in vitro for a long time. It has important research and application value in the occurrence of new mechanisms and new targets for diagnosis and treatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ARID1A gene targeted-knockout sgRNA, method for constructing ARID1A gene deletion cell strain, and application
  • ARID1A gene targeted-knockout sgRNA, method for constructing ARID1A gene deletion cell strain, and application
  • ARID1A gene targeted-knockout sgRNA, method for constructing ARID1A gene deletion cell strain, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] In order to enable those skilled in the art to better understand the solutions of the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only It is an embodiment of a part of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0033] It should be noted that the terms "first" and "second" in the description and claims of the present invention and the above drawings are used to distinguish similar objects, but not necessarily used to describe a specific sequence or sequence. It is to be understood that the data so used are interchangeable under appropriate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an ARID1A gene targeted-knockout sgRNA, a method for constructing an ARID1A gene deletion cell strain, and application, and belongs to the technical field of biological medicines. The method comprises the following steps: constructing a recombinant lentivirus plasmid ARID1A sgRNA-lentiCRISPR v2, packaging a CRISPR / Cas9 lentivirus system through 293T cells to obtain lentivirus particles, infecting a target cell strain with the lentivirus particles, and carrying out passage screening by a limited dilution method to obtain a stable cell strain with the ARID1A gene knocked out. Western blot protein expression detection shows that the protein expression of the gene knockout cell strain ARID1A is completely deleted. In addition, the multiplication capacity of the ARID1A gene knockout cell strain is obviously enhanced. The result shows that after the ARID1A gene is successfully knocked out by utilizing a CRISPR / Cas9 lentivirus system, the malignant transformation of gastric epithelial cells can be promoted.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for knocking out ARID1A gene sgRNA and constructing ARID1A gene-deleted cell lines and its application. Background technique [0002] ARID1A (AT-rich interactive domain-containing protein 1A) gene is located on human chromosome 1p35.3, about 86kb in length, including 20 exons, and encodes a protein located in the nucleus with a relative molecular weight of about 240kDa. ARID1A protein is an important subunit of the SWI / SNF chromatin remodeling complex, which participates in chromatin remodeling, such as DNA replication, transcription and damage repair, and is a key member of nuclear activities, regulating gene expression. Recent studies have found that the change of molecular genotype of chromatin remodeling complex is a new mechanism of tumorigenesis and development. The abnormal chromatin remodeling caused by the loss of ARID1A mutation leads to the di...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/113C12N15/867C12N5/10A61K31/7105A61P35/00
CPCC12N15/1135C12N15/86C12N5/0625A61K31/7105A61P35/00C12N2310/10C12N2310/20C12N2740/15043C12N2510/04
Inventor 刘希廉洁汪园园邓元李晓锋张冠军
Owner THE FIRST AFFILIATED HOSPITAL OF MEDICAL COLLEGE OF XIAN JIAOTONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com