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Electrochemical method for detecting Sudan red in food

An electrochemical and Sudan red technology, applied in the field of analytical chemistry, can solve problems that are not suitable for daily food safety supervision and detection, liver DNA structure changes, and inapplicable on-site analysis, etc., and achieve simple structure, low detection limit, Wide range of detection effects

Pending Publication Date: 2021-08-27
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term consumption of foods containing Sudan Red may change the DNA structure of the liver and cause liver diseases
[0003] Common analytical methods for Sudan Red include polarography, solid-phase extraction-gas chromatography, thin-layer chromatography, and molecularly imprinted solid-phase extraction techniques, etc., but these methods are not suitable for widespread use in daily food safety supervision and testing
In addition, based on the physical and chemical properties of Sudan Red, the sample needs to be pretreated. The pretreatment methods include normal phase solid phase extraction, liquid phase microextraction, pressurized solvent extraction, ultrasonic assisted extraction, etc. Although solid phase extraction is due to its reproducibility Good, widely used, but time-consuming, not suitable for on-site analysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An electrochemical method for detecting Sudan Red in food, specifically comprising the following steps:

[0033] (1) Preparation of expanded graphite paste electrode

[0034] Heat 90g of expanded graphite and 10g of solid paraffin to 60°C to melt for 3 minutes, stir and mix, fill one end of the glass tube after cooling, insert a copper rod at the other end to realize circuit connection, and obtain an expanded graphite paste electrode, which is ready for use;

[0035] (2) Preparation of Sudan Red Standard Solution

[0036] First weigh a certain amount of Sudan red solid, then add water to dissolve, dilute, and constant volume to obtain a series of Sudan red standard solutions with different concentrations (1 μg / L, 2 μg / L, 6 μg / L, 8 μg / L and 10 μg / L) ;

[0037] (3) Drawing of standard curve

[0038] The expanded graphite paste electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum wire electrode ...

Embodiment 2

[0044] An electrochemical method for detecting Sudan Red in food, specifically comprising the following steps:

[0045] (1) Preparation of expanded graphite paste electrode

[0046] Heat 92g of expanded graphite and 8g of solid paraffin to 65°C and melt for 2 minutes, stir and mix, fill one end of the glass tube after cooling, insert an iron rod at the other end to realize circuit connection, and obtain an expanded graphite paste electrode, which is ready for use;

[0047] (2) Preparation of Sudan Red Standard Solution

[0048] First weigh a certain amount of Sudan red solid, then add water to dissolve, dilute, and constant volume to obtain a series of Sudan red standard solutions with different concentrations (1 μg / L, 2 μg / L, 6 μg / L, 8 μg / L and 10 μg / L) ;

[0049] (3) Drawing of standard curve

[0050] The expanded graphite paste electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum wire electrode i...

Embodiment 3

[0056] An electrochemical method for detecting Sudan Red in food, specifically comprising the following steps:

[0057] (1) Preparation of expanded graphite paste electrode

[0058] Heat 95g of expanded graphite and 5g of solid paraffin to 70°C and melt for 1min, stir and mix, fill one end of the glass tube after cooling, insert an aluminum rod at the other end to realize circuit connection, and obtain an expanded graphite paste electrode, which is ready for use;

[0059] (2) Preparation of Sudan Red Standard Solution

[0060] First weigh a certain amount of Sudan red solid, then add water to dissolve, dilute, and constant volume to obtain a series of Sudan red standard solutions with different concentrations (1 μg / L, 2 μg / L, 6 μg / L, 8 μg / L and 10 μg / L) ;

[0061] (3) Drawing of standard curve

[0062] The expanded graphite paste electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum wire electrode is...

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Abstract

The invention discloses an electrochemical method for detecting Sudan red in food. The electrochemical method specifically comprises the following steps of: (1) preparing an expanded graphite paste electrode; (2) preparing a Sudan red standard solution; (3) drawing a standard curve, specifically, taking the expanded graphite paste electrode as a working electrode, a saturated calomel electrode as a reference electrode and a platinum wire electrode as a counter electrode to obtain a three-electrode system, then inserting the three-electrode system into the Sudan red standard solution, performing stirring for enriching, and performing scanning to obtain a linear regression equation, and drawing the standard curve; (4) preparing a liquid to be detected; and (5) performing detecting. The three-electrode system is simple in structure, wide in detection range and low in detection limit, can stably, quickly and accurately detect the content of the Sudan red in food, and can be repeatedly used for detecting the Sudan red.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to an electrochemical method for detecting Sudan Red in food. Background technique [0002] Sudan red is a kind of chemical dyeing agent, which is mainly used in petroleum, motor oil and some other industrial solvents for the purpose of adding color, and also for adding luster to shoes and floors. Its chemical composition contains a compound called naphthalene, which has an azo structure. Due to the nature of this chemical structure, it is carcinogenic and has obvious toxic effects on the liver and kidney organs of the human body. Because the color of food dyed with Sudan Red is very bright and not easy to fade, it can arouse people's strong appetite. Some illegal food companies add Sudan Red to food, such as chili powder, chili oil, red bean curd and red heart poultry eggs. Long-term consumption of foods containing Sudan Red may change the DNA structure of the liver a...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/308G01N27/48
Inventor 周海旭苏同超周浩宇李波高晗朱明明赵岩岩白秀芝
Owner HENAN INST OF SCI & TECH
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