Method for purifying guanosine
A purification method and technology of guanosine, applied in the field of guanosine purification, can solve the problems of low yield of guanosine, achieve the effect of improving the quality of guanosine, improving product quality, and reducing the loss of guanosine
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Embodiment 1
[0011] The fermented liquid is 85 cubic meters, the content of guanosine is 46.5g / L, under the condition of stirring, the pH value is adjusted to 9.5, steam is used to heat the fermented liquid to 96°C, and the ceramic membrane microfiltration system is filtered, the pressure is 0.2-0.5MPa, and the filtered solution, add washing water, wash the cells while filtering, after filtering, remove the cells to make bacterial protein feed, combine the washing water and dialysate, and pass through macroporous activated carbon with a pore size of 50-80nm and a particle size of 15-35 meshes Fill the column to obtain a clarified collected solution, concentrate it with a three-effect heat pump evaporator to obtain a concentrated solution with a guanosine concentration of 25.5%, adjust the pH value to 7.1 with hydrochloric acid, and then slowly cool down to 18°C within 6 hours for crystallization and separation to obtain guanosine Crude. Add the crude product into a dissolving tank filled...
Embodiment 2
[0013] The fermented liquid is 88 cubic meters, the content of guanosine is 44.3g / L. Under the condition of stirring, the pH value is adjusted to 10.3, and the steam is used to heat the fermented liquid to 95°C, and the ceramic membrane microfiltration system is filtered, and the pressure is 0.2-0.5MPa to obtain the filtered solution, add washing water, wash the cells while filtering, after filtering, remove the cells to make bacterial protein feed, combine the washing water and dialysate, and pass through macroporous activated carbon with a pore size of 50-80nm and a particle size of 15-35 meshes Fill the column to obtain a clarified collected solution, concentrate it with a three-effect heat pump evaporator to obtain a concentrated solution with a guanosine concentration of 20.1%, adjust the pH value to 6.8 with hydrochloric acid, and then slowly cool down to 17°C within 6 hours for crystallization and separation to obtain guanosine Crude. Add the crude product into a dissol...
Embodiment 3
[0015] 77 cubic meters of fermentation broth, guanosine content 47.2g / L, under stirring conditions, adjust the pH value to 11.2, heat the fermentation broth to 94°C with steam, and filter with a ceramic membrane microfiltration system at a pressure of 0.2-0.5MPa to obtain filtration solution, add washing water, wash the cells while filtering, after filtering, remove the cells to make bacterial protein feed, combine the washing water and dialysate, and pass through macroporous activated carbon with a pore size of 50-80nm and a particle size of 15-35 meshes Fill the column to obtain a clarified collected solution, concentrate it with a three-effect heat pump evaporator to obtain a concentrated solution with a guanosine concentration of 23.2%, adjust the pH value to 6.9 with hydrochloric acid, and then slowly cool down to 19°C within 6 hours for crystallization and separation to obtain guanosine Crude. Add the crude product into a dissolving tank filled with water, the solution c...
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