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Graphene magnetic nanoelectrode, electrochemical immunosensor, production method and application

An immune sensor and magnetic nanotechnology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of cumbersome and time-consuming steps, huge equipment, large sample volume, etc., achieve good conductivity, improve performance, thermal expansion The effect of a small coefficient

Active Publication Date: 2021-08-31
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above detection methods have the advantages of accuracy and high efficiency, but the steps are cumbersome and time-consuming, the sample volume is large, the equipment is huge, and the cost is high

Method used

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  • Graphene magnetic nanoelectrode, electrochemical immunosensor, production method and application
  • Graphene magnetic nanoelectrode, electrochemical immunosensor, production method and application
  • Graphene magnetic nanoelectrode, electrochemical immunosensor, production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The preparation method of graphene magnetic nanometer electrode comprises the steps:

[0044] (1) cleaning the gold electrode;

[0045] The gold electrodes were successively passed through Al with particle sizes of 0.03 μm and 0.05 μm 2 o 3 After the paste is polished, rinse it with ultrapure water, then ultrasonically wash it in ultrapure water, ethanol, and ultrapure water for 5 minutes, and place the cleaned electrode at room temperature to dry;

[0046] (2) Preparation of graphene magnetic nanoelectrodes: Graphene / Fe 3 o 4 Soak or drop-coat the nanocomposite material on the surface of the gold electrode, dry it at room temperature to form a film to obtain a modified electrode, and soak the modified electrode in nano-gold sol for 4 hours, alpha-fetoprotein antibody solution for 12 hours, and 0.25wt% bovine serum albumin solution for 30 minutes , to obtain graphene magnetic nanoelectrodes.

[0047] Preparation method of electrochemical immunosensor based on graph...

Embodiment 2

[0051] The preparation method of graphene magnetic nanometer electrode comprises the steps:

[0052] (3) cleaning the gold electrode;

[0053] The gold electrodes were successively passed through Al with particle sizes of 0.03 μm and 0.05 μm 2 o 3 After the paste is polished, rinse it with ultrapure water, then ultrasonically wash it in ultrapure water, ethanol, and ultrapure water for 5 minutes, and place the cleaned electrode at room temperature to dry;

[0054] (2) Preparation of graphene magnetic nanoelectrodes: Graphene / Fe 3 o 4 Soak or drip-coat the nanocomposite material on the surface of the gold electrode, dry it at room temperature to form a film to obtain a modified electrode, and soak the modified electrode in nano-gold sol for 5 hours, alpha-fetoprotein antibody solution for 10 hours, and 0.25wt% bovine serum albumin solution for 45 minutes , to obtain graphene magnetic nanoelectrodes.

Embodiment 3

[0056] The preparation method of graphene magnetic nanometer electrode comprises the steps:

[0057] (4) cleaning the gold electrode;

[0058] The gold electrodes were successively passed through Al with particle sizes of 0.03 μm and 0.05 μm 2 o 3 After the paste is polished, rinse it with ultrapure water, then ultrasonically wash it in ultrapure water, ethanol, and ultrapure water for 5 minutes, and place the cleaned electrode at room temperature to dry;

[0059] (3) Preparation of graphene magnetic nanoelectrode: the Graphene / Fe 3 o 4 Soak or drop-coat the nanocomposite material on the surface of the gold electrode, dry it at room temperature to form a film to obtain a modified electrode, and soak the modified electrode in nano-gold sol for 6 hours, alpha-fetoprotein antibody solution for 9 hours, and 0.25wt% bovine serum albumin solution for 40 minutes , to obtain graphene magnetic nanoelectrodes.

[0060] Embodiment 1-3 of the present invention has all made the graphe...

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Abstract

The invention belongs to the technical field of electrochemical immunosensors, and particularly relates to a graphene magnetic nanoelectrode, an electrochemical immunosensor, a production method and an application. The production method of the graphene magnetic nanoelectrode comprises the following steps: cleaning a gold electrode; and modifying the surface of the gold electrode with a Graphene / Fe3O4 nano composite material, airing at room temperature to form a film to obtain a modified electrode, and sequentially soaking the modified electrode in nano gold sol for 4-6 hours, 0.01-100 ng.mL <-1 > alpha fetoprotein antibody solution for 9-12 hours and 0.25 wt% bovine serum albumin solution for 30-45 minutes. According to the invention, a magnetic nano-composite constructed by graphene is constructed on the surface of the gold electrode, and the electrochemical immunosensor is produced based on the graphene magnetic nano-electrode. The electrochemical immunosensor is high in sensitivity, strong in antibody adsorption capacity, wide in detection concentration range and suitable for detection of immune protein.

Description

technical field [0001] The invention belongs to the technical field of electrochemical immunosensors, and relates to graphene magnetic nanocomposites, electrochemical immunosensors, preparation methods and applications. Background technique [0002] Immunoglobulin (immunoglobulin) refers to animal proteins with antibody activity, among which alpha-fetoprotein is the abbreviation of alpha fetoprotein (ERAFP), and AFP is an acidic glycoprotein produced by liver cells in the early stage of embryonic development. AFP in normal adult serum is about 10ng·mL -1 , while in the serum of most primary liver cancer and teratoma patients, the content of alpha-fetoprotein is high, and AFP has a high positive rate for the diagnosis of digestive tract malignant tumors and lung cancer. It is important to diagnose HCC. At present, the detection methods of alpha-fetoprotein mainly use enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), indirect hemagglutination, agar double di...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30G01N27/327G01N27/38G01N33/53
CPCG01N27/48G01N27/308G01N27/3278G01N27/38G01N33/53
Inventor 朱宇萍吕晓玲
Owner NEIJIANG NORMAL UNIV
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