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Kit for detecting folic acid content of human erythrocytes, detection method and application

A technology for detecting human body and detection method, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of expensive instruments, complex detection operations of detection kits, and expensive detection instruments, and achieve the effect of avoiding complex operations.

Active Publication Date: 2021-08-31
天津康博尔生物基因技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a test kit, detection method and application for detecting the folic acid content of human red blood cells, which solves the shortcomings of the detection kits in the prior art that the detection operation is complicated or the detection equipment is expensive, and fills the gap in the detection of red blood cells by immunochromatography. The blank of folic acid content is more in line with the needs of folic acid screening in the population, and is suitable for the promotion and application of grassroots units or general resource areas and institutions
After long-term research, the inventor found that by using the most common immunochromatography technology at present, by screening antibodies and optimizing sample processing reagents, the provided kit can achieve simple, low-cost, and rapid detection of folic acid content in red blood cells, effectively solving the problem. Existing methods are either complex in operation or expensive in equipment, filling the gap in the determination of folic acid content in red blood cells by immunochromatography

Method used

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  • Kit for detecting folic acid content of human erythrocytes, detection method and application
  • Kit for detecting folic acid content of human erythrocytes, detection method and application
  • Kit for detecting folic acid content of human erythrocytes, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of immunochromatography test paper card in human erythrocyte folic acid detection kit

[0032] (1) Preparation of NC membrane detection area

[0033] Cut the nitrocellulose detection membrane into long strips, place it on the platform of the dot-spraying apparatus, and spray the folic acid-BSA and goat anti-mouse on the detection membrane respectively to form T-line and C-line. Dry at 37°C for 2h.

[0034] (2) Preparation of conjugation pads

[0035] Take 200 μg of latex microspheres and 4 μg of folic acid monoclonal antibody, in MES buffer, one-step chemical coupling for 2 hours, and then resuspend in PBS buffer to make a marker. Cut the glass wool into long strips, place it on the platform of the dot sprayer, take 90 μL of the marker, spray it on the glass wool, and dry at 37°C for 2 hours.

[0036] (3) Assembly of test strips

[0037] On the support plate of the test strip, there are sample pads, binding pads, NC membrane detection area and w...

Embodiment 2

[0038] Embodiment 2: quantitative curve preparation test

[0039] (1) The test paper card and immunochromatographic analyzer (model: KBE-6000) prepared in Example 1 were selected.

[0040] (2) Remove the assembled test paper card, use 8, 16, 32, 64, 128, and 256 ng / mL folic acid calibrator respectively, and measure with the test paper card to obtain the strength value of each calibrator.

[0041] (3) Test results: if image 3 As shown, take the logarithmic value of the calibrator concentration as the X-axis, and take the intensity value as the Y-axis, draw a scatter diagram, and perform linear analysis, R 2 >0.98.

Embodiment 3

[0042] Embodiment 3: precision test

[0043] (1) Using the test paper card and the calibration system of Example 2, a precision test is carried out on the test paper card of the present invention.

[0044] (2) Test the reference substance at two concentration levels (80 ng / mL, 200 ng / mL), and repeat the test 10 times.

[0045] (3) Test results: if Figure 4 As shown, after the low-value reference product and the high-value reference product were repeatedly measured 10 times, the mean, standard deviation and CV were calculated. The results showed that the low-value reference product CV was 9.7%, and the high-value reference product CV was 12.1%.

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Abstract

The invention relates to a kit for detecting folic acid content of human erythrocytes, a detection method and application. The invention provides a kit for detecting folic acid content of human erythrocytes. The kit comprises a whole blood sample treatment reagent, a precipitation solution and an immunochromatography test paper card, wherein the whole blood sample treatment reagent comprises an antioxidant, a surfactant and a reaction buffer solution, the immunochromatography test paper card comprises a supporting plate, a sample pad, a combination pad, a nitrocellulose membrane detection area and a water absorption pad are sequentially pasted on the supporting plate, and a marked folic acid monoclonal antibody is adsorbed on the combination pad. By adopting an immunochromatography technology, screening antibodies, optimizing sample treatment reagents and the like, the provided kit can realize the effect of simply and quickly detecting the folic acid content of the red blood cells at low cost, the defects of complicated operation or expensive instruments of the existing means are effectively overcome, and the blank of measuring the folic acid content of the red blood cells by using the immunochromatography method is filled.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a kit, a detection method and an application for detecting the folic acid content of human red blood cells. Background technique [0002] Folic acid is a water-soluble vitamin that is necessary for the growth and reproduction of human cells. It can be used to treat anemia caused by folic acid deficiency, and it is also a nutritional supplement for pregnant women. More than 50% of neonatal neural tube defects (neural tube defects, NTDs) cases are related to folic acid deficiency in early pregnancy, folic acid supplementation can significantly reduce the incidence of NTDs. Long-term reasonable supplementation of folic acid can also help reduce the risk of cardiovascular and cerebrovascular diseases. [0003] Serum folic acid is considered to be an indicator of recent folic acid nutritional status, and serum folic acid levels alone cannot distinguish betwee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/54313
Inventor 张亮杨光
Owner 天津康博尔生物基因技术有限公司
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