The invention provides an activatable
signal amplification detection technology based on fluorescent dye
tyramine and application of the activatable
signal amplification detection technology in nucleocapsid
protein detection. The problems of insufficient detection sensitivity, poor specificity, loud
background noise and the like in a traditional
tyramine signal amplification technology are solved by adopting a dual amplification detection technology (combining with an
aptamer or an
antibody to improve specificity and utilizing
tyramine to realize
signal amplification) capable of activating tyramine
signal amplification and ROS response; and the detection difficulty caused by the own characteristics of the nucleocapsid
protein is avoided. According to the technology, the sensitivity, specificity and accuracy of detection are remarkably improved, different
virus nucleocapsid proteins can be effectively distinguished,
background noise is reduced, a reliable method is provided for SARS-CoV-2 nucleocapsid
protein detection, meanwhile, a basis is provided for researching the influence of different structures on the detection effect, and the technology has wide application prospects in the fields of
clinical diagnosis and scientific research.