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A kind of preparation method of erythrine

A technology for erythromycin and extract, which is applied in the field of preparation of erythromycin, can solve the problems of low extraction rate, complicated process, long time-consuming and the like, and achieves the effects of high extraction rate and simple operation.

Active Publication Date: 2022-05-03
WUYI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the related art, the preparation process of erythrine is time-consuming, the process is complicated, and the extraction rate is low; therefore, it is necessary to develop a method for the preparation of erythrine, which has a high extraction rate.

Method used

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  • A kind of preparation method of erythrine
  • A kind of preparation method of erythrine

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Experimental program
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Effect test

Embodiment 1

[0058] The present embodiment is a kind of preparation method of erythrine, comprising the following steps:

[0059] S1. Extraction:

[0060] Get Niu Dali medicinal material (Guangdong Mailinke Biotechnology Co., Ltd., Dianbai base) to dry, pulverize, pass through a 100-mesh sieve, dry, get 680g fine powder, and configure a volume concentration of 70% ethanol aqueous solution, according to the ratio of material to liquid 1: 15g / mL, reflux extraction twice at 80°C. Ethanol was recovered by rotary evaporation to obtain 100 g of the crude extract, and the content of erythrine in the crude extract was determined to be 1.50% according to the above-mentioned high-performance liquid chromatography.

[0061] S2, extraction:

[0062] Add an appropriate amount of distilled water to the above crude extract to dissolve (the ratio of solid to liquid is 1:1 to suspend the crude extract) to obtain a suspension; extract at a volume ratio of 1:1 between the suspension and the extractant; It w...

Embodiment 2

[0076] The present embodiment is a kind of preparation method of erythrine, comprising the following steps:

[0077] S1. Extraction:

[0078] Take Niu Dali medicinal material (Guangdong Mailinke Biotechnology Co., Ltd., Fengkai base) to dry, crush, weigh 1600g, prepare a volume concentration of 70% ethanol aqueous solution, according to the ratio of material to liquid 1:15g / mL, at room temperature Soak and extract for 3 days, stir twice a day, and extract 3 times in total. The soaking liquids were combined and evaporated to dryness to recover ethanol to obtain 230 g of crude extract; the content of erythracine in the crude extract was determined to be 0.41% according to the above-mentioned high performance liquid chromatography.

[0079] S2, extraction:

[0080] Take 230g of the crude extract, add an appropriate amount of distilled water to dissolve, and obtain a suspension (the ratio of solid to liquid is 1:1); extract according to the volume ratio of the suspension and the...

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Abstract

The invention discloses a preparation method of erythrine, which comprises the following steps: S1, extracting oxalicine with alcohol, separating solid and liquid to obtain a crude extract; S2, dissolving the crude extract in water and using it; Extraction with solvent I and organic solvent II, collecting the organic layer extracted by organic solvent II; removing the organic solvent to obtain a fine extract; S3, after dissolving the fine extract in water, purifying it with a non-polar macroporous resin, and then sequentially using water and ethanol aqueous solution of different concentrations in the second section for elution; the collected eluent was concentrated and dried to obtain purified extract I; S4, after dissolving the purified extract I with methanol aqueous solution I, purified by gel column, and then eluted with The eluate was collected and concentrated to dry to obtain purified extract II; S5, after dissolving the purified extract II with methanol aqueous solution II, washing after crystallization, and collecting the solid phase to obtain erythrine. The extraction method of the invention has high extraction rate and high product purity.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to a preparation method of erythrine. Background technique [0002] Niu Dali is the dry root of Callerya speciosa Schot (Callerya speciosa Schot), a leguminous plant of the genus Millettia, formerly known as Millettia speciosa Champ. Niu, Paishanhu, Xueteng, Admiralty Root, Dumping Admiralty, Sweet Niu Dali, etc. are well-known medicinal and edible medicinal materials in Lingnan area. It contains active ingredients such as erythrine, formononetin, and sophorin, among which the content of erythrine is relatively high. [0003] Hypaphorine, also known as Hypaphorine or Hypaphorine, chemical name: N,N,N-trimethyltryptophan, molecular formula: C 14 h 18 N 2 o 2 , CAS No.: 487-58-1, is an indole alkaloid. Erythrine has anti-inflammatory, anti-bone loss, inhibition of adipocyte differentiation and other activities. [0004] In the related art, the preparation process of er...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 李冬利黄毅祺翟擎峰冼燕英莫金凤邹子铭谢寅正吴盼盼洪为谦甘礼社张焜
Owner WUYI UNIV