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Method for degrading polyethylene glycol terephthalate through combination of bacteria and enzymes

A technology combining polyethylene terephthalate and bacterial enzyme, which is applied in the direction of plastic recycling and recycling technology, can solve the problems of affecting enzyme activity and reducing the pH value of the system.

Active Publication Date: 2021-09-03
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the enzymatic degradation products of PET also have a certain inhibitory effect on the degradation process.
It was found that the enzymatic degradation intermediates of PET, bis(2-hydroxyethyl) terephthalate (BHET) and 2-hydroxyethyl monoterephthalate (MHET), were competitive inhibitors of PET degrading enzymes , while MHET is further hydrolyzed to TPA much slower than BHET, and the accumulation of the degradation product TPA will lead to a decrease in the pH value of the system, thereby affecting the activity of the enzyme

Method used

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  • Method for degrading polyethylene glycol terephthalate through combination of bacteria and enzymes
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  • Method for degrading polyethylene glycol terephthalate through combination of bacteria and enzymes

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Embodiment 1

[0033] Screening and Identification of Example 1 Bacterial Strains

[0034] 1. Experimental materials and methods

[0035] 1.1 Medium:

[0036] LB liquid medium (g / L): yeast extract (5.0), peptone (10.0), NaCl (10.0), solvent is water, adjusted to pH 7.0, sterilized at 115°C for 30 minutes;

[0037] LB solid medium is 20g / L agar added to LB liquid medium.

[0038] BSM medium (i.e. basic inorganic salt medium, g / L): K 2 HPO 4 ·3H 2 O(1.0), NaCl(1.0), (NH 4 ) 2 SO 4 (0.5), MgSO 4 ·7H 2 O(0.4), CaCl 2 (0.0755), FeCl 3 ·6H 2 O(0.0143), the solvent is water, adjusted to pH 7.5, sterilized at 115°C for 30min;

[0039] Add 20g / L agar to BSM solid medium.

[0040] TPA liquid medium: add terephthalic acid (TPA) (dissolved in DMSO, stock solution concentration is 250mg / mL) in BSM medium, make the final concentration of TPA in the solution be 250mg / L.

[0041] TPA solid medium: add 20g / L agar to TPA liquid medium.

[0042] 1.2 Screening of TPA degrading bacteria

[0043] E...

Embodiment 2

[0066] 1. Experimental materials

[0067] Klebsiella variicola SY1 (Klebsiella variicola SY1), preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2020198, the preservation date is June 10, 2020, the address is Wuhan, China, Wuhan University, postcode 430072 .

[0068] Candida antarctica cutinase HiC (Novozym 51032) was purchased from Chengdu Ainahua Chemical Co., Ltd., with a nominal esterase activity of 15kU / g and a protein content of 1.424mg / mL; lipase CALB (Candida antarctica lipaseB, CALB) Purchased from Beijing Gao Ruisen Technology Co., Ltd., the nominal esterase activity is 5kU / g, and the protein content is 1.8mg / mL.

[0069] lcPET (low-crystallinity PET, purchased from Shaoxing Xiangyu Green Packaging Co., Ltd.), is a plastic particle of 3.0mm×2.5mm×2.0mm, with a crystallinity of 11.62%.

[0070] boPET (biaxially oriented PET, purchased from Shaoxing Xiangyu Green Packaging Co., Ltd.), is a plastic film with a thickness of...

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Abstract

The invention discloses a method for degrading polyethylene glycol terephthalate through combination of bacteria and enzymes. According to the method, a reaction system is formed by using a cell suspension obtained by carrying out amplification culture on Klebsiella variicola SY1 and Candida antarctica cutinase HiC as catalysts, using polyethylene glycol terephthalate as a substrate and using a buffer solution with the pH value of 7.0-9.0 as a reaction medium, a degradation reaction is carried out at 30-60 DEG C to degrade the high polymer PET into a monomeric compound; through combined application of bacteria and enzymes, product inhibition of PET degrading enzymes is relieved, and the degradation efficiency is improved.

Description

[0001] (1) Technical field [0002] The invention relates to a method for combining bacteria and enzymes to degrade polyethylene terephthalate. [0003] (2) Background technology [0004] Polyethylene terephthalate (PET) is a polyester of terephthalic acid (TPA) and ethylene glycol. It is also one of the most widely used thermoplastic polyester materials, accounting for about 1 / 5 of the output, the massive accumulation of its waste has caused serious environmental problems. At present, the treatment of PET waste is mainly divided into physical method, chemical method and biological method. The physical recycling method first classifies the recycled PET plastics, and after mechanical processing into pieces, they are further washed and dried, and then remanufactured into granules by heating and other processes. Although physical recycling is widely used, this method often leads to PET molecular chain breakage, molecular weight reduction, and impurity components that cannot be r...

Claims

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Application Information

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IPC IPC(8): C08J11/10C08L67/02
CPCC08J11/105C08J2367/02Y02W30/62
Inventor 邱乐泉吴石金李彤彤尹辛格
Owner ZHEJIANG UNIV OF TECH
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