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Intelligent adjustable self-heating inoculator for microbial experiments

An adjustable and microbiological technology, applied in the field of microbiological experiments, can solve the problems that liquids are prone to bursting, danger, and high temperature of alcohol lamps, and achieve the effects of avoiding splashing of bacteria-containing liquids, visualizing temperature changes, and avoiding mistakes.

Active Publication Date: 2021-09-03
QINGDAO BINHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the whole process, the experimenter cannot accurately know the temperature of the inoculation loop, which may easily lead to the death of bacteria due to improper operation by the experimenter; the temperature of the alcohol lamp is high during the use of the alcohol lamp, and the liquid on the inoculation loop is prone to burst when heated , causing danger; if a mistake occurs when taking bacteria, there will be too many bacteria on the inoculation loop, and repeated operations will waste time

Method used

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  • Intelligent adjustable self-heating inoculator for microbial experiments
  • Intelligent adjustable self-heating inoculator for microbial experiments
  • Intelligent adjustable self-heating inoculator for microbial experiments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Put the solid slant medium cultured with Escherichia coli and the triangular flask with liquid medium in the left hand, turn on the heating switch of the inoculation loop and the switch of the display screen, and use the ring finger and little finger of the right hand to hold the tampon and triangular flask on the slant Bottle of tampon. Stop heating when the temperature of the display screen reaches 900°C; wait for the temperature of the inoculation loop to drop to room temperature, take the bacteria on the slope, put the bacteria into the liquid medium of the Erlenmeyer flask, and recover the tampons of the flask and the slope. The Erlenmeyer flask was shaken at 37°C for 12 hours on a shaker at 200 rpm.

[0041] (2) Separation by dashes. Turn on the heating switch of the inoculation loop and the switch of the display screen, and stop heating when the temperature of the display screen 12 reaches 300°C. Open the bacterial solution that has been cultivated on the s...

Embodiment 2

[0046] (1) Put the solid slant medium cultured with Escherichia coli into the left hand and separate by marking. Turn on the heating switch of the inoculation loop and the switch of the display screen, and stop heating when the temperature of the display screen 12 reaches 300° C., and wait until the temperature of the inoculation loop drops to room temperature.

[0047] (2) Shift the gear of push button 8 to third gear, put the inoculation loop part into the solid culture medium of Escherichia coli, and obtain the agar containing Escherichia coli. Then, switch the push button 8 gears to the first gear, draw a line continuously on the flat plate of the solid medium, cover the culture dish after marking, and invert the culture. This experiment was repeated three times.

[0048] (3) Repeat the above steps (1)-(2) three times using a common inoculation loop.

[0049] (4) After culturing in a constant temperature incubator at 37°C for 12-24 hours, discontinuous single colonies ca...

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Abstract

The invention discloses an intelligent adjustable self-heating inoculator for microbial experiments. The inoculator comprises a head inoculating loop, a sliding rod and a handle which are connected in sequence, wherein the head inoculating loop comprises an inoculating needle and two spliced inoculating loops; the inoculating needle is mounted at the top of the sliding rod; a sliding block track and two sliding blocks are arranged in the sliding rod; the bottoms of the spliced inoculating loops are connected with the sliding blocks; a gear shifting mechanism comprises a push button, a guide rail sliding block and two connecting rods; the guide rail sliding block is arranged at the handle; the push button is assembled on the guide rail sliding block in a sliding manner; the handle comprises a constant-temperature heating device and a temperature controller which are connected with a battery through wires; the constant-temperature heating device is used for heating and adjusting the temperature of the head inoculating loop; and the temperature controller electrically connected with the constant-temperature heating device is used for monitoring the environment temperature and the overall temperature of the head inoculating loop. The inoculator is simple to operate and low in cost, and can meet the requirements of various microbial experiments.

Description

technical field [0001] The invention relates to the technical field of microbial experiments, in particular to an intelligent adjustable self-heating inoculum for microbial experiments. Background technique [0002] The inoculation loop is a commonly used inoculation tool in bacterial culture. It is widely used in many disciplines such as microbial detection, cell microbiology, and molecular biology. It is an indispensable laboratory tool. Especially when cultivating microorganisms, it is necessary to use the inoculation loop to pick the strains for inoculation. [0003] In the general microbial experiment process, the inoculation loop needs to be burnt on the alcohol lamp until all the bacteria on the inoculation loop are dead, and then wait for it to cool down before proceeding to the next step. However, during the whole process, the experimenter cannot accurately know the temperature of the inoculation loop, which may easily lead to the death of bacteria due to improper ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/38C12M1/34C12M1/12C12M1/02
CPCC12M33/02C12M37/00C12M41/12
Inventor 张竞竞范洪勇张维芬陈嘉怡
Owner QINGDAO BINHAI UNIV
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