Fluorescence labeling test strip for rapid detection of food-borne pathogenic bacteria and preparation method of fluorescence labeling test strip

Abstract

The invention discloses a fluorescence labeling test strip for rapid detection of food-borne pathogenic bacteria and a preparation method thereof, a novel fluorescence labeling test strip is constructed by using up-conversion fluorescence and immunochromatography technologies, and rapid detection and discrimination of the food-borne pathogenic bacteria are realized. The method comprises steps of connecting food-borne pathogenic bacterium specific antibody with a fluorescent substance to form a signal probe, and spraying the signal probe on a specific glass fiber membrane; taking the food-borne pathogenic bacterium specific antibody and a secondary antibody corresponding to the food-borne pathogenic bacterium resisting antibody as capture probes, and scribing on a nitrocellulose membrane to form a detection line (T line) and a quality control line (C line); and assembling the treated glass fiber membrane, the nitrocellulose membrane and the PVC bottom plate to obtain the fluorescence labeling test strip. A to-be-detected substance dropwise added on a sample pad of the test strip can be combined with the signal probe and can be combined with the corresponding antibody and the second antibody on the T line and the C line again in the lateral flowing process, the strip appearance condition at the corresponding position is observed through fluorescence imaging equipment so as to judge whether the food-borne pathogenic bacteria exist or not, and then rapid diagnosis of the food-borne pathogenic bacteria is achieved.

Description

technical field [0001] The invention belongs to the technical field of pathogenic bacteria detection, and in particular relates to constructing more than one test strip that converts nanoparticles into fluorescent markers to realize rapid, sensitive and highly specific detection of food-borne pathogenic bacteria. Background technique [0002] Foodborne diseases have become a global public health safety problem, which are often caused by ingestion of food contaminated with pathogenic microorganisms and their toxins or other chemicals. Foods in which foodborne bacteria are affected can cause illness and death in severe cases. [0003] Humans are suitable hosts for food-borne pathogens, and malnourished young children, the elderly and immunocompromised persons are more susceptible. According to reports, there are about 14,000 cases of shigellosis every year, and bacillary dysentery caused by Shigella is mainly transmitted through the digestive tract. In order to monitor the o...

AI Technical Summary

Problems solved by technology

However, PCR-based methods require complex cell disruption and nucleic acid extraction protocols

Therefore, rapid and real-time detection of foodborne pathogens is limited to some extent

[0005] On the whole, although the traditional detection method has low technical requirements and low cost, it is time-consuming and laborious, and the detection is inaccurate. Although the real-time quantitative PCR method overcomes some shortcomings of the traditional method, it has high cost and high operational technical requirements. still takes a long time

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