Preparation method and application of composition containing fermented rapeseed oil

A technology of rapeseed oil and composition, applied in the field of preparation of fermented rapeseed oil composition, capable of solving environmental and human harmful problems

Inactive Publication Date: 2021-09-10
如薇化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem of harmfulness to the environment and human body existing in anti-hair loss products in the prior art, a p

Method used

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  • Preparation method and application of composition containing fermented rapeseed oil
  • Preparation method and application of composition containing fermented rapeseed oil
  • Preparation method and application of composition containing fermented rapeseed oil

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Experimental program
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Effect test

Embodiment 1

[0025] 1) The preparation process of fermented rapeseed oil: After the strains are activated, they are cultured on a shaker with YM Broth medium. 0.01% of magnesium sulfate, 0.01% of calcium chloride dihydrate, 0.01% of sodium chloride, 0.01% of peptone, 2% of rapeseed oil, etc. are formulated for fermentation. The fermentation conditions are: pH range 3.5-4.0, fermentation temperature 28 ℃, speed 110rpm, fermentation time 48 hours, ventilation 0.5vvm. After the fermentation is finished, sterilize and stand still to collect the supernatant, and filter to obtain fermented rapeseed oil.

[0026] 2) Extraction of oriental cypress leaves, after crushing the oriental cypress leaves, adopt hot water extraction process, the mass ratio of solid to liquid is: 1:15, the extraction temperature is 75°C, the extraction time is 4 hours, and then the extraction time is 2500r / Centrifuge at a high speed for 1 min, collect the supernatant, concentrate under reduced pressure at 70°C, and separ...

Embodiment 2

[0033] 1) The preparation process of fermented rapeseed oil: After the strains are activated, they are cultured on a shaking table with YM Broth medium. 0.05% of magnesium sulfate, 0.05% of calcium chloride dihydrate, 0.01% of sodium chloride, 0.05% of peptone, 4% of rapeseed oil, etc. are formulated for fermentation. The fermentation conditions are: pH range 3.7-4.3, fermentation temperature 28 ℃, speed 110rpm, fermentation time 48 hours, ventilation 0.5vvm. After the fermentation is finished, sterilize and stand still to collect the supernatant, and filter to obtain fermented rapeseed oil.

[0034]2) Extraction of oriental cypress leaves, after pulverizing the oriental cypress leaves, adopt the hot water extraction process, the mass ratio of solid to liquid is: 1:20, the extraction temperature is 75°C, the extraction time is 4 hours, and then the extraction time is 2800r / Centrifuge at a high speed for 1 min, collect the supernatant, concentrate under reduced pressure at 70...

Embodiment 3

[0041] 1) The preparation process of fermented rapeseed oil: After the strain is activated, it is cultured on a shaking table with YM Broth medium. 0.05% of magnesium sulfate, 0.05% of calcium chloride dihydrate, 0.05% of sodium chloride, 0.1% of peptone, 6% of rapeseed oil, etc. are made into the medium for fermentation. The fermentation conditions are: pH value range 3.5-4.0, fermentation temperature 29 ℃, speed 110rpm, fermentation time 56 hours, ventilation 0.6vvm. After the fermentation is finished, sterilize and stand still to collect the supernatant, and filter to obtain fermented rapeseed oil.

[0042] 2) Extraction of oriental cypress leaves, after pulverizing the oriental cypress leaves, adopt hot water extraction process, the mass ratio of solid to liquid is: 1:25, the extraction temperature is 80°C, the extraction time is 4 hours, and then the extraction time is 3000r / Centrifuge at a high speed for 1 min, collect the supernatant, concentrate under reduced pressur...

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Abstract

The invention provides a preparation method of a composition containing fermented rapeseed oil. The preparation method comprises the following steps that a strain is activated, shake cultivation is carried out by using a YM Broth culture medium, a nutrient solution, rapeseed oil and the like are added to prepare a culture medium for fermentation after the culture is finished, sterilizing, standing and supernate collecting are carried out after the fermentation is finished, and filtering is carried out to obtain the fermented rapeseed oil; cacumen biotae, polygonum multiflorum, ginger, ginseng and rosemary are correspondingly extracted and purified to obtain corresponding extracts; and the fermented rapeseed oil is mixed with cacumen biotae, polygonum multiflorum, ginger, ginseng, rosemary extract, butanediol and water according to a certain proportion, and sterilizing and filtering are carried out to obtain the composition containing fermented rapeseed oil. Compared with a traditional anti-hair-loss chemical reagent, the composition containing fermented rapeseed oil can prevent hair loss, nourish and blacken hair and obviously improve the hair loss phenomenon of a patient, is superior to a fat-removing hair-growing liquid in the aspects of improving head oil and promoting new hair growth, has no burden on the body and no pollution to the environment, can be used safely and trustingly, and is suitable for the fields of beauty, hair care and the like.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation and cosmetics, in particular to a preparation method and application of a composition containing fermented rapeseed oil. Background technique [0002] The relevant records of hair loss were first seen in "Emperor's Inner Diameter", in which hair loss is called "hair loss", "hair loss" and "mao fold". There is also a record of hair loss in "Lingshu Tiannian": "At the age of forty, the internal organs and twelve meridians are all prosperous to calm down, and the interstitial lines begin to thin out, the hair is sagging, and the temples are gray." Jin Dynasty The title of "hair baldness" appeared in "Elbow Reserve Emergency Prescription" written by Ge Hong. At this time, hair loss is mostly recorded as a symptom of the disease. After the founding of the People's Republic of China, the economy and science and technology developed rapidly. In the excellent situation of national policy ...

Claims

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Application Information

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IPC IPC(8): A61K8/9761A61K8/9789A61K8/9794A61K8/92C12P7/64C12N1/16A61P17/14A61Q7/00A61Q5/02A61Q5/00C12R1/72
CPCA61K8/9761A61K8/9789A61K8/9794A61K8/922A61Q7/00A61Q5/002A61Q5/02A61P17/14A61Q5/008C12P7/6463C12N1/16A61K2800/85A61K2800/78
Inventor 陈秀玉王绍梅秦涛郭星星詹柱刘晓磊丁冲冲赵治勇何沪宝
Owner 如薇化妆品有限公司
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