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Composite microbial preparation and preparation method thereof

A technology of compound microorganisms and preparations, applied in the field of biological preparations, can solve the problems of single strains with single disease prevention mechanism, low control effect and instability

Pending Publication Date: 2021-09-10
HAINAN JINYUFENG BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the research on biological control agents is a single strain, and CN103131657A "A Bacillus subtilis and its biological control preparation and application" discloses the application of Bacillus subtilis in biological control preparations, but the disease prevention mechanism of a single bacterial strain is single, The control effect is low and unstable
However, due to the existence of multiple bacterial strains in the composite bacterial preparation, the preservation and application of the composite bacterial preparation are limited by various conditions.

Method used

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  • Composite microbial preparation and preparation method thereof

Examples

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Effect test

Embodiment 1

[0030] A composite microbial preparation, comprising: Bacillus subtilis, Streptomyces roseus and diatomaceous earth, the CFU ratio of the Bacillus subtilis and Streptomyces roseus is 5:2.

[0031] The amount of live bacteria contained in the compound microbial preparation is 10 8 cfu / g.

[0032] A preparation method for a composite microbial preparation, comprising the following steps:

[0033] (1) Inoculate Bacillus subtilis and Streptomyces roseus into PDA medium respectively, activate and cultivate at 28°C for 6h, and carry out activation culture, then inoculate Bacillus subtilis into the fermentation medium of Bacillus subtilis, at 32-35 Cultivate for 72 hours under conditions of pH 7 to obtain a fermentation broth of Bacillus subtilis; inoculate Streptomyces roseus flavum into a Streptomyces roseus flavum fermentation medium, and culture at 30-32°C for 144 hours to obtain a fermentation broth of Streptomyces roseus flavum;

[0034] Every liter of the Bacillus subtilis f...

Embodiment 2

[0040] A compound microbial preparation, comprising: Bacillus subtilis, Streptomyces roseus flavum and mica powder, wherein the CFU ratio of the Bacillus subtilis and Streptomyces roseus flavum is 4:1.

[0041] The amount of live bacteria contained in the compound microbial preparation is 10 6 cfu / g.

[0042] A preparation method for a composite microbial preparation, comprising the following steps:

[0043] (1) Inoculate Bacillus subtilis and Streptomyces roseus into PDA medium respectively, activate and cultivate at 28°C for 6h, and carry out activation culture, then inoculate Bacillus subtilis into the fermentation medium of Bacillus subtilis, at 32-35 Cultivate for 72 hours under conditions of pH 7 to obtain a fermentation broth of Bacillus subtilis; inoculate Streptomyces roseus flavum into a Streptomyces roseus flavum fermentation medium, and culture at 30-32°C for 144 hours to obtain a fermentation broth of Streptomyces roseus flavum;

[0044] Every liter of the Bacil...

Embodiment 3

[0050] A composite microbial preparation, comprising: Bacillus subtilis, Streptomyces roseus and diatomaceous earth, the CFU ratio of the Bacillus subtilis and Streptomyces roseus is 5:2.

[0051] The amount of live bacteria contained in the compound microbial preparation is 10 8 cfu / g.

[0052] A preparation method for a composite microbial preparation, comprising the following steps:

[0053] (1) Inoculate Bacillus subtilis and Streptomyces roseus into PDA medium respectively, activate and cultivate at 28°C for 6h, and carry out activation culture, then inoculate Bacillus subtilis into the fermentation medium of Bacillus subtilis, at 32-35 Cultivate for 72 hours under conditions of pH 7 to obtain a fermentation broth of Bacillus subtilis; inoculate Streptomyces roseus flavum into a Streptomyces roseus flavum fermentation medium, and culture at 30-32°C for 144 hours to obtain a fermentation broth of Streptomyces roseus flavum;

[0054] Every liter of the Bacillus subtilis fer...

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Abstract

The invention provides a composite microbial preparation and a preparation method thereof. Bacillus subtillis and streptomyces roseoflavus are compounded into the composite microbial preparation, and the control effect is achieved through various disease prevention mechanisms such as resistance substance secretion, hyperparasitism, induction of host cells to generate a defense structure and competitive action and the like; and the two strains also have a synergistic effect, can effectively and stably prevent and treat soil-borne disease pathogenic bacteria such as gray mold, powdery mildew, banana wilt and soybean root rot, and can also be used for soil improvement after continuous cropping of crop fields such as vegetables, flowers, melons and fruits, forest trees, Chinese herbal medicines and the like.

Description

technical field [0001] The invention relates to the technical field of biological preparations, in particular to a composite microbial preparation and a preparation method thereof. Background technique [0002] At present, the prevention and control of plant diseases is mainly based on chemical control. However, long-term use of chemical control agents will not only cause pollution, but also lead to increasing resistance to various diseases. The probiotics in the plant and the soil are destroyed, and the immune system of the plant is destroyed, making it more difficult to control planting diseases. [0003] In recent years, due to the increasingly serious pollution of chemical pesticides, biological control has attracted increasing attention due to its advantages of no pollution, no residue, difficulty in producing drug resistance, and safety for the ecological environment and human health. The application in prevention and treatment has attracted much attention. Biologica...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01N63/28A01P3/00C09K17/14C12R1/125C12R1/465C09K101/00
CPCC12N1/20A01N63/22A01N63/28C09K17/14C09K2101/00Y02W30/40
Inventor 解晓燕
Owner HAINAN JINYUFENG BIOLOGICAL ENG
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