Preparation method of casuarina tanning and hypoglycemic application of same

A casuarina and tannin technology, which is applied in the field of separation and purification of natural products and hypoglycemic applications, can solve the problems that α-glucosidase inhibitory activity and hypoglycemic applications have not been reported, and achieve large sample processing capacity and high inhibitory activity , good repeatability

Inactive Publication Date: 2021-09-24
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation method of casuarina tannin and its α-glucosidase inhibitory activity and hypoglycemic use have not been reported

Method used

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  • Preparation method of casuarina tanning and hypoglycemic application of same
  • Preparation method of casuarina tanning and hypoglycemic application of same
  • Preparation method of casuarina tanning and hypoglycemic application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Wash 1 kg of earthworms, add 70% (volume ratio of ethanol to water: 70:30) ethanol containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid 1:8 (w / v, g / mL) Aqueous solution (the volume ratio of ethanol to water is 70:30), ultrasonic extraction at 40°C in the dark for 60min, then vacuum filtration, repeated extraction of the filter residue once, combined filtrates, and vacuum rotary evaporation at 45°C to remove ethanol, to obtain Nim extract crude extract.

[0048] Add the same volume of ethyl acetate to the crude extract of Direnzo extract for extraction, extract 4 times, collect the ethyl acetate phase, then concentrate it by rotary evaporation in vacuum at 45°C, blow with nitrogen, and obtain the ethyl acetate extract powder of Direnzo , the liquid chromatogram of the ethyl acetate extract powder of Dirennan is shown in figure 1 shown.

[0049] Use the preparative liquid phase column Unitary C18 20mm×250mm, dissolve the powder of ethyl a...

Embodiment 2

[0053] Wash 5 kg of earthworms, add 70% (volume ratio of ethanol to water: 70:30) ethanol containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid 1:8 (w / v, g / mL) Aqueous solution (the volume ratio of ethanol to water is 70:30), ultrasonic extraction at 40°C in the dark for 60min, then vacuum filtration, repeated extraction of the filter residue once, combined filtrates, and vacuum rotary evaporation at 45°C to remove ethanol, to obtain Nim extract crude extract.

[0054] Add the same volume of ethyl acetate to the crude extract of Direnzo extract for extraction, extract 4 times, collect the ethyl acetate phase, then concentrate it by rotary evaporation in vacuum at 45°C, blow with nitrogen, and obtain the ethyl acetate extract powder of Direnzo .

[0055] Use the preparative liquid phase column Unitary C18 20mm×250mm, dissolve the powder of ethyl acetate extract of Direnzo in DMSO to make the concentration reach 40mg / mL, inject it into the prepara...

Embodiment 3

[0058] Wash 10kg of earthworms, add 70% (volume ratio of ethanol to water: 70:30) ethanol containing 0.1% (v / v) hydrochloric acid according to the ratio of material to liquid 1:8 (w / v, g / mL) Aqueous solution (the volume ratio of ethanol to water is 70:30), ultrasonic extraction at 40°C in the dark for 60min, then vacuum filtration, repeated extraction of the filter residue once, combined filtrates, and vacuum rotary evaporation at 45°C to remove ethanol, to obtain Nim extract crude extract.

[0059] Add the same volume of ethyl acetate to the crude extract of Direnzo extract for extraction, extract 4 times, collect the ethyl acetate phase, then concentrate it by rotary evaporation in vacuum at 45°C, blow with nitrogen, and obtain the ethyl acetate extract powder of Direnzo .

[0060] Use the preparative liquid phase column Unitary C18 20mm×250mm, dissolve the powder of ethyl acetate extract of Direnzo in DMSO to make the concentration reach 60mg / mL, inject it into the prepara...

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Abstract

The invention discloses a preparation method of casuarina tanning and hypoglycemic application of casuarina tanning. The preparation method comprises the steps of alcohol extraction and concentration, ethyl acetate extraction, high-pressure preparative liquid chromatography purification and high-speed counter-current chromatography separation. High-pressure preparative liquid chromatography and high-speed counter-current chromatography are combined, and then technological parameters are optimized, therefore, high-purity casuarina tanning monomer is separated and prepared from a melastoma dodecandrum raw material with complex substance composition. An activity test shows that the casuarina tannin monomer can significantly inhibit the activity of alpha-glucosidase, and can be used for preparing an alpha-glucosidase inhibitor to control the postprandial blood sugar level.

Description

technical field [0001] The present invention relates to the fields of separation and purification of natural products and hypoglycemic applications, in particular to a preparation method of casuarina tannin and its application in the preparation of α-glucosidase inhibitors. Background technique [0002] Diabetes is one of the common chronic diseases worldwide. Diabetic patients are usually accompanied by symptoms of hyperglycemia. Long-term hyperglycemia can cause damage to tissues and organs such as nerves, heart, blood vessels and kidneys, and cause a variety of acute and chronic complications. . According to the statistics of the World Health Organization (WHO), there were 422 million diabetic patients in the world in 2014, of which type 2 diabetes is the most common. In recent years, with the changes in people's eating habits and lifestyles and the acceleration of the aging process, the prevalence of diabetes in my country has risen rapidly. By the end of 2016, the numb...

Claims

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Application Information

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IPC IPC(8): C07D493/08A61K36/185A61P3/10
CPCC07D493/08A61K36/185A61P3/10
Inventor 陈卫徐阳艾哈迈德·K·拉什旺
Owner ZHEJIANG UNIV
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