Novel visual pathogen nucleic acid rapid detection chip

Abstract

The invention discloses a novel visual pathogen nucleic acid rapid detection chip which comprises a substrate and a flow path sheet. The substrate and the flow path sheet are sealed into one whole body; the substrate is composed of a bottom plate, a USB interface, a heating resistor and a thermosensitive element; the flow path sheet is a communicating pipeline structure composed of a buffer solution hole, a sample adding hole, a reaction region, a detection region and a waste liquid vessel; the detection region consists of a chromatography test paper card; a long-chain nucleic acid probe I, a long-chain gold-labeled nucleic acid probe II, a short-chain gold-labeled nucleic acid probe III and a short-chain gold-labeled nucleic acid probe IV are fixed on the chromatography test paper card; and the USB interface can be connected with a mobile phone charger and the like. According to the novel visual pathogen nucleic acid rapid detection chip, rapid visual detection of new coronavirus pathogens in a saliva sample is implemented, the saliva sample is taken, a lysis solution is added for lysis, RNA is extracted through a paramagnetic particle method, fluid is controlled through the liquid driving force of a buffer solution, the one-step reaction is performed, and visual detection of results is implemented within one hour without any instrument.

Description

technical field [0001] The invention relates to the field of integrated microfluidic chip manufacturing technology and gene diagnosis technology, in particular to a novel visual pathogen nucleic acid rapid detection chip. Background technique [0002] Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a novel in vitro isothermal amplification of specific nucleic acid fragments. It is simple, rapid and specific. Currently, RT-LAMP has been used in clinical pathogenic microorganisms. The fields of detection, genetic disease diagnosis, SNP typing, infectious disease surveillance, and identification of genetically modified foods have shown great potential and are gradually being used more and more widely. [0003] After the combination of RT-LAMP technology and microfluidic chip is realized, the whole detection process only includes one step of manual operation: sample addition operation, which can avoid the problems caused by some personnel mistakes, sing...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a new type of rapid detection chip for visual pathogenic nucleic acid to solve the problem that the detection of respiratory pathogenic nucleic acid proposed in the above background technology requires a corresponding instrument detection system, which cannot realize visualization and cannot reach the portability of POCT detection. asked questions

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