Surface plasmon imaging system based on differential denoising

Abstract

The invention discloses a surface plasmon imaging system based on differential denoising. The surface plasmon imaging system comprises a rotary illumination system, a tube mirror, a beam splitter, an oil immersion microscope objective, a gold film substrate, a high-precision displacement platform and an imaging light path, and a surface plasmon has the characteristics of local field enhancement and sensitivity to environment refractive index change, and can be used for imaging small particles. The system comprises the following steps: firstly, performing rotary illumination on an object by using incident light by using a rotary illumination system, eliminating interference fringes generated by the incident light and scattered light, and obtaining an image without the interference fringes and with a smaller size; and on the basis, using a differential denoising method to quickly move a high-precision displacement platform, so that the background noise of the image is removed, and the signal-to-noise ratio of imaging is improved. According to the imaging system, the signal-to-noise ratio of surface plasmon microimaging is greatly improved by using a differential denoising method, high-contrast imaging can be carried out on nanoparticles with the diameter as low as 22 nm, and the particle size resolution is 10 nm.

Description

technical field [0001] The invention relates to the field of surface optical microscopic imaging with high signal-to-noise ratio, in particular to a surface plasmon imaging based on differential denoising. Background technique [0002] Optical detection of individual nanoparticle size has been widely used in biophysical research and is expanding to applications including national security, environmental monitoring, and early diagnosis. This technique provides an important means of probing the fundamental properties of biological and photonic materials and has revolutionized our understanding of biophysical phenomena that occur at the nanoscale. Nanoparticles with smaller diameters tend to be more harmful to human health than larger nanoparticles, as they can penetrate deep into the lungs, where they may then induce lung cancer, respiratory diseases and even organ dysfunction. Therefore, we should focus more on the detection of ultrafine particles, especially nanoparticles w...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of low signal-to-noise ratio of the traditional surface plasmon microscopy imaging system, and propose a surface plasmon imaging system based on differential denoising, which can detect particles with a diameter as low as 22nm Nanoparticles for high signal-to-noise ratio imaging with 10nm particle size resolution

Images