Multifunctional microscopic imaging slide glass based on optical thin film

Abstract

The invention discloses a multifunctional microscopic imaging slide based on an optical thin film. The multifunctional microscopic imaging slide comprises a photonic crystal incident layer, a scattering thin film layer, a photonic crystal functional layer and a sample layer, the layers can be prepared layer by layer in a film layer processing mode, and can also be respectively prepared and then combined by utilizing refractive index matching oil. According to the invention, multiple one-dimensional photonic crystal composite structures prepared through special design are utilized for the first time, vertical incident light is converted into large-numerical-aperture emergent light, total internal reflection light and surface plasmon, and high-contrast dark field and surface plasmon imaging is successfully achieved on a traditional microscope.

Description

technical field [0001] The invention relates to the field of high-contrast dark-field optical microscopic imaging, in particular to the field of multifunctional microscopic imaging slides based on optical films. Background technique [0002] Microscopic technology is the most direct means for people to understand the microscopic world. Optical microscopy technology presents the image of the microscopic world directly in front of our eyes. It is the most intuitive and commonly used microscopic technology among all microscopic technologies. Dark-field microscopy technology reduces the collection of illumination light and enhances the collection of scattered light after illumination in the process of optical microscopy imaging to improve the signal-to-noise ratio of the imaging system. According to the numerical aperture of the illumination light, the dark field microscope can provide illumination methods such as transmission and total internal reflection. The above-mentioned ...

AI Technical Summary

Problems solved by technology

[0006] The technical problem of the present invention is to overcome the disadvantages of traditional dark-field microscopes, such as complex optical structure, high use cost and poor convenience, and provide a multi-functional microscopic imaging slide based on optical film. Structure, using the vertically incident illumination light of the traditional microscope system, realizes multiple functions including large numerical aperture transmission, total reflection dark field and surface plasmon illumination for one or more working wavelengths at the same time, reducing the cost of the dark field microscope system cost of use and improve ease of use

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