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Application of isoliquiritigenin in preparation of anti-neuroinflammation drugs

A neuroinflammation and isoliquiritigenin technology, applied in the field of medicine, can solve problems such as side effects and rarely effective reversal of the disease

Pending Publication Date: 2021-10-15
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many compound drugs for the treatment of neuroinflammation, but these drugs can only improve the symptoms, seldom can effectively reverse the disease, and even have side effects, so it is particularly important to find new drugs and methods for the treatment of neuroinflammation

Method used

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  • Application of isoliquiritigenin in preparation of anti-neuroinflammation drugs
  • Application of isoliquiritigenin in preparation of anti-neuroinflammation drugs
  • Application of isoliquiritigenin in preparation of anti-neuroinflammation drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Water maze test to test the effect of isoliquiritigenin on the improvement of LPS-induced neuroinflammation and memory impairment in mice

[0027] In this embodiment, the experimental animals were randomly divided into 5 groups, namely: normal control group (Control), LPS group (LPS, 250 μg / kg / d), positive drug group (LPS+positive drug, TTP488, 5 mg / kg / day) , low-dose isoliquiritigenin group (LPS+low-dose isoliquiritigenin, ISL-L, 25mg / kg / day) and high-dose isoliquiritigenin group (LPS+high-dose isoliquiritigenin, ISL-H, 50mg / kg / day) , 7 in each group. In the first week, each experimental group was administered according to the concentration, and then injected with LPS. In the second week, each experimental group was dosed according to the concentration every day. After 2 weeks, the memory function of the mice was investigated using the water maze experiment. The experimental data were expressed as Mean ± SD. All experimental data were statistically analyzed...

Embodiment 2

[0029] Example 2 The effect of isoliquiritigenin on the inflammatory response in the brain tissue of mice with neuroinflammation induced by LPS

[0030] The experimental grouping and dosing scheme of this example are the same as in Example 1. After the administration, the mice were sacrificed, and the complete brain tissue was taken out to make paraffin sections, and immunofluorescence was performed on the sections. For the results, please refer to image 3, and then immunofluorescent staining was used to measure the expression of the inflammatory factor Iba-1 in the hippocampus. The experimental data were expressed as Mean±SD. All the experimental data were statistically analyzed using GraphPad Prism 8 software. The parameters between groups of mice in each group were expressed by ANOVA test, P <0.05 was considered to be statistically different.

[0031] The main steps of making paraffin sections include cleaning the tissue and fixing it in 4% paraformaldehyde for 1 week → ri...

Embodiment 3

[0035] Example 3 Transcriptome sequencing (RNA-seq) analysis of gene expression differences before and after isoliquiritigenin treatment of LPS-induced neuroinflammation in mice

[0036] Total RNA was extracted before and after isoliquiritigenin treatment of LPS-induced neuroinflammation in mice according to the instructions, and the qualified total RNA samples (including biological replicates) were sent to BGI for transcriptome sequencing (RNA-seq) analysis. After preprocessing the raw sequencing data, the effective transcriptome data of each sample before and after isoliquiritigenin treatment of LPS-induced neuroinflammation in mice was obtained.

[0037] Figure 4 It is a graph of sequencing results of mouse brain tissue transcriptome in Example 3, wherein (A) is a Venn diagram of gene expression between groups of Control group, LPS group, and ISL group, and RNA-seq data analysis shows that Control group, LPS group, ISL group The number of co-expressed genes was 21497, the...

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Abstract

The invention belongs to the technical field of medicines, and relates to application of isoliquiritigenin. The invention provides the application of the isoliquiritigenin, and experimental results show that the isoliquiritigenin can safely and effectively control neuroinflammatory response, so that the cognitive function, dysmnesia and motor coordination ability of brain tissues are improved, and the isoliquiritigenin can be used for preventing and treating Alzheimer's disease, Parkinson's disease and the like. The isoliquiritigenin is applied to preparation of medicines or health-care products for preventing and treating neuroinflammation, and has good application value and development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to the application of isoliquiritigenin. Background technique [0002] Inflammatory response is a complex cascading process for the body to resist infection and injury. Its pathological changes are characterized by excessive production of inflammatory factors, extensive infiltration of inflammatory cells, and tissue necrosis and disintegration. Neuroinflammation is a special immune response that occurs in the nervous system. . Under normal conditions, microglia and astrocytes are inactive and play a role in maintaining normal tissue homeostasis in the central nervous system. During brain infection or injury, these cells are activated to launch the immune response and tissue repair processes, and once the infection or injury has recovered, the cells return to a quiescent state. Under normal circumstances, inflammation will disappear soon, but if inflammation continues chronically, it...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P25/00A61P25/28A61P25/16A61P25/24A61P9/10A61K36/486
CPCA61K31/122A61P25/00A61P25/28A61P25/16A61P25/24A61P9/10A61K36/486
Inventor 李雪彤郑希张蓝月李春莲谭泽楷蔡秋仰吴显仪张伟诚赵凯黄泽彬
Owner GUANGDONG UNIV OF TECH
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