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Brevibacillus brevis ADT and application thereof

A technology of Bacillus brevis and strains, applied in the direction of application, bacteria, and other chemical processes, can solve the problems of microbial survival state, unstable action state, low treatment efficiency, and inability to achieve purification effects, etc.

Active Publication Date: 2021-10-22
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems to be solved in the actual application process. The main problem is that these microorganisms enter the environment and easily compete with the indigenous microorganisms in the environment. The survival state and function state of microorganisms in the environment are unstable. In the application process, a stable purification effect cannot be achieved, resulting in low governance efficiency and no corresponding governance effect, which hinders its application and promotion

Method used

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  • Brevibacillus brevis ADT and application thereof
  • Brevibacillus brevis ADT and application thereof
  • Brevibacillus brevis ADT and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and Identification of Brevibacillus brevis ADT

[0024] 1. Isolation of strains

[0025] The strains were extracted from endives grown on both sides of the Dianbaigou river bank in Outing Street, Longhu District, Shantou City:

[0026] (1) Surface disinfection: Wash the collected fresh and healthy chicory samples with floating water on the surface, take about 3g of leaf samples and rinse them with sterile water, soak them in 1% sodium hypochlorite solution for 5 minutes, and use sterile water to clean them. Rinse 3-5 times, then soak in 75% ethanol solution for 5 minutes, rinse 3-5 times with sterile water, and dry the water with sterilized filter paper.

[0027] (2) Treatment: Cut the surface-sterilized samples into small pieces with sterilized scissors, soak them in 15ml of sterile water for 20min, and grind them into a homogenate fully in a sterilized mortar.

[0028] (3) Separation: Spread the ground homogenate evenly on the NA medium plate, then place t...

Embodiment 2

[0043] Example 2 Water body of a certain river in Qingdao

[0044] (1) Characteristics of the water body: The water body of the river course has serious eutrophication, and a large area of ​​cyanobacteria blooms broke out. The dominant cyanobacteria species is Microcystis aeruginosa. The sample was taken back to the laboratory for testing, and the COD content was 89.71mg / L, and the ammonia nitrogen content was 7.05mg / L.

[0045] (2) Processing method:

[0046] (2.1) Take 1 mL of sterile water (control group), 1 mL of Bacillus brevis ADT (experimental group 1), 1 mL of Bacillus subtilis (experimental group 2) fermentation liquid (experimental group 2) and add 1000 mL of the in the river sample solution.

[0047] Among them, Bacillus subtilis was purchased from Shanghai Ruichu Biotechnology Co., Ltd.

[0048] The above-mentioned strains were inoculated in NA liquid medium after being activated on the plate according to the conventional method, and cultured with shaking at 30°C ...

Embodiment 3

[0056] Example 3 Water body of a certain river in Shantou

[0057] Water characteristics: The river course is a severe black and odorous water body, the water color is gray and white, and the smell is foul. The water sample was taken for microscopic observation. The types of microorganisms in the water body are extremely rare, and there are a large number of long rod-shaped bacteria. The COD content of the water body is 185.60mg / L, and the ammonia nitrogen content It is 64.24mg / L.

[0058] Approach:

[0059] (2.1) Take 1 mL of sterile water (control group), 1 mL of Bacillus brevis ADT (experimental group 1), 1 mL of Bacillus licheniformis (experimental group 2) fermentation liquid (experimental group 2) and add 1000 mL of the in the river sample solution.

[0060] Among them, Bacillus licheniformis was purchased from Shanghai Ruichu Biotechnology Co., Ltd.

[0061] The above-mentioned strains were inoculated in NA liquid medium after being activated on the plate, and cultur...

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Abstract

The invention belongs to the field of microbial environment restoration, and particularly relates to Brevibacillus brevis ADT and application thereof in water pollutant degradation, algae inhibition and saline-alkali soil improvement. The Brevibacillus brevis ADT is preserved in the China Center for Type Culture Collection on May 17, 2021, the preservation address is Wuhan, China, the accession number is CCTCC NO: M 2021537, and the Brevibacillus brevis ADT is classified and named as Brevibacillus brevis ADT. The strain has good effects in the aspects of degrading water pollutants, inhibiting algae and improving saline-alkali soil, and is not reported and utilized at home and abroad.

Description

technical field [0001] The invention belongs to the field of microbial environment restoration, and in particular relates to a Bacillus brevis ADT and its application in degrading water pollutants, inhibiting algae and improving saline-alkali soil. Background technique [0002] Bacillus brevis belongs to the genus Bacillus, which widely exists in water, air, soil and plants. It has the advantages of fast growth, simple nutrition, and strong stress resistance. It can produce active substances such as gramicidin and chitinase, which are beneficial to A variety of bacteria and fungi have inhibitory effect. Due to the antibacterial properties of the secretion of Bacillus brevis, the current research and application of Bacillus brevis mainly focus on the control of plant diseases, and there are relatively few researches and applications on it in other fields. [0003] The use of bacillus-like microorganisms has been widely used in the process of environmental treatment, such as ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C09K17/14C12R1/01C02F101/16C02F101/30C09K109/00
CPCC12N1/20C02F3/34C09K17/14C02F2101/16C02F2101/30C09K2109/00
Inventor 刘宗丽李佳霖郭静静刘正一吕丽娜张健王勇丁海兵秦松
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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