Working electrode containing metal-organic framework and preparation method and application thereof
A technology of working electrodes and organic frameworks, which is applied in the field of working electrodes containing metal-organic frameworks and its preparation, can solve the problems of low loading capacity and loading fastness of three-dimensional flower-like proteins, cumbersome preparation process, and lack of electrical conductivity. Achieve the effect of improving electrochemical sensing performance, good repeatability, good sensitivity and flexibility
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[0029] A method for preparing a working electrode containing a metal-organic framework provided by the invention comprises the following steps:
[0030] S1. Prepare a layer of metal layer on the surface of the substrate;
[0031] S2. Prepare protein phosphate buffer solution with pH≤7;
[0032] S3. Immerse the substrate treated in step S1 in the protein phosphate buffer solution described in step S2 to react for a preset time, and the metal layer on the surface of the substrate reacts under acidic conditions to obtain metal ions, which then react with protein and phosphate The working electrode containing the metal-organic framework is obtained.
[0033] The metal layer is a simple metal or a metal oxide, preferably a simple metal. The metal layer slowly releases metal ions under acidic conditions, and the metal ions are chelated with protein and phosphate to obtain a multidimensional structure metal-protein chelate. When it is a metal element, the metal element in the meta...
Embodiment 1
[0042] A working electrode containing a metal-organic framework, prepared by the following steps:
[0043] S1. Surface metallization of conductive materials
[0044] Clean the carbon fiber to remove surface stains. Then roughen, sensitize, and activate in sequence.
[0045] Cleaning method: put carbon fiber into ethanol solution, and ultrasonically clean it for 1-2 minutes.
[0046] Coarsening: impregnate the cleaned carbon fiber in 5M NaOH solution for roughening, reaction temperature: 30°C, reaction time: 30min, take it out, wash it, and dry it;
[0047] Sensitization: use 40ml / L HCl as solvent to prepare 10g / L SnCl 2 2H 2 O sensitization solution, immerse the roughened carbon fiber in the sensitization solution, reaction temperature: 30°C, reaction time: 30min.
[0048] Activation: Prepare 0.5g / L PdCl with 20ml / L HCl as solvent 2 Activation solution, immerse the sensitized carbon fiber in the activation solution, reaction temperature: 30°C, reaction time: 30min.
[004...
Embodiment 2
[0056] A working electrode containing a metal-organic framework. Compared with Example 1, the difference is that the carbon fibers in step S1 are replaced with stainless steel metal fibers. Others are substantially the same as in Embodiment 1, and will not be repeated here.
[0057] see figure 2 As shown, it can be seen that another shape of metal-enzyme multidimensional structure material has grown on the surface of the stainless steel metal fiber. Therefore, the structure of the metal-enzyme chelate can also be regulated by regulating the type of substrate.
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