Integrated vitrified freezing/resuscitation instrument

Abstract

The invention relates to the technical field of embryo culture technology, and discloses an integrated vitrified freezing/resuscitation instrument which comprises a workbench, a reagent containing mother port system, a transmission system, an automatic liquid injection system, a liquid nitrogen transportation system, a partition temperature control system and a computer. Wherein multiple rows of treatment systems are arranged on the workbench, liquid nitrogen storage tanks corresponding to the treatment systems are further arranged on the workbench, and each treatment system comprises multiple groups of reagent tanks which are arranged at intervals. According to the multi-group treatment system, a plurality of sample freezing or resuscitation programs can be simultaneously carried out, the working efficiency is high, and is controlled by a computer to program work. Compared with the prior art, work is more automatic and intelligent, manual operation is replaced, the problems caused by manual operation in actual work can be effectively avoided, and meanwhile the problems of liquid nitrogen pollution and sample cross infection in existing open type freezing can be solved.

Description

technical field [0001] The invention relates to the technical field of embryo freezing / resuscitation, in particular to an integrated vitrification / resuscitation program instrument. Background technique [0002] At present, during the treatment of infertility patients in the reproductive center, about 80% of the patients need to cryopreserve the redundant embryos, and at the appropriate time in the follow-up treatment, the embryos are resuscitated and then transferred. Vitrification / resuscitation has better clinical outcomes and has become a routine option in reproductive centers. [0003] At this stage, both embryo freezing and thawing need to rely on two experienced embryologists to use a stereo microscope to process them in reagents with different gradient concentrations. In the specific operation process, there will be the following problems: [0004] (1) Differences in technical proficiency lead to out-of-control time management of different personnel in actual operati...

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Problems solved by technology

[0004] (1) Differences in technical proficiency lead to out-of-control time management of different personnel in actual operation, and uneven storage volume of liquid in freezing, which manifests as large differences in clinical treatment outcomes, which further affects the quality control of vitrification technology;

[0005] (2) In order to prevent the risk of embryo confusion, during the actual freezing process of embryos, different patients need to be operated separately, especially in most reproductive centers in my country with more than 1000 cycles, the workload is huge, and the long-term tension will aggravate the risk of medical errors;

[0006] (3) In the traditional manual operation process, due to the open design of the freezing carrier, there is also the risk of sample loss during the freezing recovery process;

[0007] (4) Due to the open storage design, there is inevitably liquid nitrogen pollution and the possibility of cross-contamination between different samples;

[0008] (5) At present, frozen resuscitation reagents basically rely on imports. Due to the great differences in the temperature of different brands of technical solutions and the length of operation steps, etc., which affect the operation experience, the staff mostly rely on a single brand, and a single product has a supply chain risk for medical treatment.

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