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Preparation method of broccoli bud seedling extract and anti-inflammatory activity of broccoli bud seedling extract

A technology of broccoli sprouts and extracts, which can be used in anti-inflammatory agents, drug combinations, non-central analgesics, etc. Effect

Pending Publication Date: 2021-10-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research focuses on the antioxidant, antibacterial and anticancer activities of broccoli, and there is little research on the anti-inflammatory activity.

Method used

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  • Preparation method of broccoli bud seedling extract and anti-inflammatory activity of broccoli bud seedling extract
  • Preparation method of broccoli bud seedling extract and anti-inflammatory activity of broccoli bud seedling extract
  • Preparation method of broccoli bud seedling extract and anti-inflammatory activity of broccoli bud seedling extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Harvest fresh broccoli sprouts cultivated with slightly acidic electrolyzed water (Slightly Acidic Electrolyzed Water, SAEW), freeze-dry, and crush to 80 mesh sieves to obtain broccoli sprout dry powder.

[0034] 2) Add n-hexane to the dry powder obtained in the step 1) according to the ratio of solid to liquid 1:8, the unit is g / mL; after shaking on a shaker at room temperature for 1 h, centrifuge at 5000 r / min for 10 min to obtain the residue, and place it in a fume hood Allow organic reagents to dry.

[0035] 3) Take the residue obtained in step 2), add distilled water, place in a dark environment, and hydrolyze at room temperature for 8 hours; the solid-liquid ratio of broccoli sprout dry powder to distilled water is 1:4, and the unit is g / mL.

[0036] 4) adding NaCl and Na in the wet meal of described step 3) 2 SO 4 A desiccant composed of NaCl and Na 2 SO 4 The mass ratio of the desiccant added is 4:3, and the mass ratio of the distilled water in the step 3...

Embodiment 2

[0039] 1) Harvest fresh broccoli sprouts cultivated in tap water (Tap Water, TW), freeze-dry, and crush to 80-mesh sieve to obtain dry broccoli sprout powder.

[0040] 2) Add n-hexane to the dry powder obtained in the step 1) according to the ratio of solid to liquid 1:8, the unit is g / mL; after shaking on a shaker at room temperature for 1 h, centrifuge at 5000 r / min for 10 min to obtain the residue, and place it in a fume hood Allow organic reagents to dry.

[0041] 3) Take the residue obtained in step 2), add distilled water, place in a dark environment, and hydrolyze at room temperature for 8 hours; the solid-liquid ratio of broccoli sprout dry powder to distilled water is 1:4, and the unit is g / mL.

[0042]4) adding NaCl and Na in the wet meal of described step 3) 2 SO 4 A desiccant composed of NaCl and Na 2 SO 4 The mass ratio of the desiccant added is 4:3, and the mass ratio of the step 3) distilled water is 2:1. Add dichloromethane and vortex mix, ultrasonically e...

Embodiment 3

[0045] The beneficial effects of the present invention are further described below through the protective effect of the broccoli sprout extract prepared by the present invention on inflammatory cells and colitis mice:

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PUM

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Abstract

The invention relates to the fields of food and natural active substance extraction, in particular to a preparation method of a broccoli bud seedling extract, which comprises the steps of broccoli bud seedling drying, crushing, degreasing, hydrolysis, ultrasonic extraction and rotary evaporation, wherein dichloromethane is used for extracting active ingredients of broccoli buds and seedlings. The broccoli bud seedling extract extracted by the preparation method disclosed by the invention shows relatively good anti-inflammatory activity and has a very high application value.

Description

technical field [0001] The invention relates to the field of food and the field of extraction of natural active substances, in particular to a preparation method of broccoli sprout extract and its anti-inflammatory activity. Background technique [0002] Broccoli (Brassica oleracea L.var.italica), also known as broccoli, is an annual plant of the genus Brassica in the family Brassicaceae. Broccoli is rich in natural active ingredients, such as glucosinolates (GLS for short), polyphenols, anthocyanins, etc., and its nutrient content ranks among the top plants. When glucosinolates are contacted with myrosinase (Myrosinase, MYR), they can be hydrolyzed to generate isothiocyanates (ITCs) with significant anticancer effects. The glucoraphanin with the highest content in broccoli is glucoraphanin (Glucoraphanin, GRA), and its hydrolyzate - sulforaphane (Sulforaphane), is by far the most powerful natural ingredient in anticancer effect. [0003] Sprout vegetables refer to the use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07C331/20A61P1/00A61P29/00
CPCC12P13/00C07C331/20A61P1/00A61P29/00
Inventor 刘海杰孙瑜阳刘军
Owner CHINA AGRI UNIV
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