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Polygonatum fructooligosaccharide and preparation and application thereof

A technology of fructooligosaccharides and polygonatum fruit, applied in the field of natural phytochemistry, can solve the problem of less pharmacological research on Polygonatum fructooligosaccharides

Pending Publication Date: 2021-10-29
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current researches focus on Polygonatum polysaccharides, and there are few pharmacological studies on Polygonatum fructooligosaccharides (PFOS)

Method used

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  • Polygonatum fructooligosaccharide and preparation and application thereof
  • Polygonatum fructooligosaccharide and preparation and application thereof
  • Polygonatum fructooligosaccharide and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation of embodiment 1 fructooligosaccharide

[0031] Fresh Polygonatum rhizome (6.0kg) was cut into thin slices (2×2mm thick), and soaked in 95% ethanol (18L) at 25-30°C for 10 days with intermittent stirring to degrease, the extraction was repeated twice, and the The residue was air-dried (yield 4.85 kg) and extracted three times with boiling distilled water (30 L each, 3 hours each). Then, the extracts were combined and concentrated to one-tenth of the volume, and then separated by ultrafiltration using 8K Da and 1K Da cut-off membranes to obtain low molecular weight Polygonatum fructo-oligosaccharide PFOS (8K Da-1 K Da). After concentration, PFOS was dried under vacuum at 50°C.

Embodiment 2

[0032] The preparation of embodiment 2 fructooligosaccharides

[0033] Cut the fresh Polygonatum rhizome (6.0kg) into small slices (2×2mm thick), add 12L of petroleum ether at 50°C for reflux extraction for 2 hours, filter, evaporate the filter residue to dry the solvent, add 12L of 2mol / L NaOH solution, at 50°C, 100W ultrasonic extraction for 1h, cold filtration, the filtrate was concentrated, the extracts were combined, concentrated to one-tenth of the volume, and then separated by ultrafiltration using 8K Da and 1K Da cut-off membranes to obtain low molecular weight Polygonatum fructooligosaccharides PFOS . After concentration, PFOS was dried under vacuum at 50°C.

Embodiment 3

[0034] The preparation of embodiment 3 fructooligosaccharides

[0035] Cut the fresh Polygonatum rhizome (6.0kg) into small slices (2×2mm thick), add 18L distilled water, add 250g cellulase, enzymolyze at 50°C for 2 hours, boil to kill the enzyme, cool, filter, concentrate the filtrate, and extract The mixtures were combined and concentrated to one-tenth of the volume, and then separated by ultrafiltration using 8K Da and 1K Da cut-off membranes to obtain the low molecular weight Polygonatum fructo-oligosaccharide PFOS. After concentration, PFOS was dried under vacuum at 50°C.

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Abstract

The invention provides polygonatum fructooligosaccharide hydrogel. An active ingredient of the polygonatum fructooligosaccharide hydrogel comprises polygonatum fructooligosaccharide. By the research, the invention finds that the polygonatum fructooligosaccharide (PFOS) has the effect of promoting wound healing. Based on the research, the invention actually provides application of the polygonatum fructooligosaccharide used as the active ingredient in preparation of a product for promoting wound healing.

Description

technical field [0001] The present invention relates to the field of natural phytochemistry. Background technique [0002] Rhizoma Polygonatum (PCH) belongs to Liliaceae, and is a plant with the same source of medicine and food. For the treatment and prevention of cough, fatigue, dizziness and respiratory problems. PCH contains a variety of active ingredients, which have the functions of anti-aging, anti-tumor, anti-bacterial, anti-virus, lowering blood sugar, blood lipid, enhancing immunity, and improving memory. Therefore, the research of PCH and the development of new dosage forms are of great value. [0003] It has been reported that PCH is rich in polysaccharide compounds (PCHP). Previous studies have shown that PCHP has an anticancer effect on human cervical cancer Hela cells. The researchers used four PCHPs to study their effects on HeLa cell proliferation, cytotoxicity, caspase-3 activity, cell cycle, and apoptosis in vitro. The results showed that with the incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/702A61K45/06A61K9/06A61K47/32A61K47/38C13K11/00A61P17/02
CPCA61K31/702A61K45/06A61K9/06A61K47/32A61K47/38A61K9/0014A61P17/02C13K11/00
Inventor 张志锋王甜甜熊中立
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES