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Polyphenol nano-reactor and preparation method and application thereof

A nano-reactor and polyphenol technology, applied in the field of biomedicine, can solve the problems of affecting enzyme activity and lack of conformational freedom, and achieve the effects of reaction control, easy reaction and high repeatability

Pending Publication Date: 2021-10-29
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, tightly encapsulated enzymes in host materials often lack conformational freedom, which affects enzyme activity

Method used

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  • Polyphenol nano-reactor and preparation method and application thereof
  • Polyphenol nano-reactor and preparation method and application thereof
  • Polyphenol nano-reactor and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A preparation method of a polyphenol nanoreactor, comprising the steps of:

[0034] (1) Preparation of GOx / CAT@ZIF nanoparticles:

[0035] Dissolve 0.25mg GOx, 0.25mg CAT and 115mg 2-methylimidazole in 3mL deionized water and mix well, then quickly add 2.5mL 2.376mg / mL zinc nitrate hexahydrate pre-cooled at 4℃, and place the above solution Stir the reaction at 4°C for 30 min, and when the solution turns milky white, use an ultrafiltration tube to concentrate the solution, take the upper concentrated solution and wash it repeatedly with deionized water to remove excess GOx and unreacted precursors, and freeze-dry at -20°C to obtain GOx / CAT@ZIF nanoparticles. The morphology of nanoparticles was characterized by transmission electron microscopy, such as figure 1 As shown, the shape of GOx / CAT@ZIF nanoparticles is regular, the particle size is uniform, and the particle size is about 90nm;

[0036] (2) Preparation of GOx / CAT@TA-PEI nanoreactor:

[0037]Weigh 60mg of TA p...

Embodiment 2

[0043] A preparation method of a polyphenol nanoreactor, comprising the steps of:

[0044] (1) Preparation of GOx@ZIF nanoparticles:

[0045] Add 1 mg of GOx and 460 mg of 2-methylimidazole into 12 mL of deionized water and mix evenly, then quickly add 10 mL of 2.376 mg / mL zinc nitrate hexahydrate pre-cooled at 4 °C, and place the above solution at 4 °C for 30 min. After turning milky white, use an ultrafiltration tube to concentrate the solution, take the upper concentrated solution and wash it repeatedly with deionized water to remove excess GOx and unreacted precursors, freeze-dry to obtain GOx@ZIF nanoparticles;

[0046] (2) Preparation of GOx@TA-PEI nanoreactor:

[0047] Weigh 60mg of TA powder, add 3ml of pure water, vortex fully, ultrasonicate, configure 20mg / mL TA mother solution, take 400μL and add it to 80mL Tris-HCl solution (50mM, pH 8.0), and then take the prepared in step (1) 12 mg of GOx@ZIF nanoparticles were dispersed in the above-mentioned Tris-HCl solution...

Embodiment 3

[0053] A preparation method of a polyphenol nanoreactor, comprising the steps of:

[0054] (1) Preparation of GOx / CAT@ZIF nanoparticles:

[0055] Add 0.5mg GOx, 0.5mg CAT and 230mg 2-methylimidazole into 6mL of deionized water and mix well, then quickly add 5mL of 2.376mg / mL zinc nitrate hexahydrate pre-cooled at 4°C, and place the above solution at 4°C Stir and react for 30 minutes. After the solution turns milky white, use an ultrafiltration tube to concentrate the solution. Take the upper concentrated solution and wash it repeatedly with deionized water to remove excess GOx and unreacted precursors to obtain GOx / CAT@ZIF nanoparticles;

[0056] (2) Preparation of GOx / CAT@TA-PEI nanoreactor:

[0057] Weigh 60mg of TA powder, add 6ml of pure water, vortex fully, ultrasonicate, configure TA mother solution of 10mg / mL, take 400μL and add it to 40mL Tris-HCl solution (50mM, pH 8.0), and then take the prepared in step (1) GOx / CAT@ZIF nanoparticles were dispersed in the above-men...

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Abstract

The invention provides a polyphenol nano-reactor and a preparation method and application thereof. The polyphenol nano-reactor is prepared by comprising the following steps that enzyme is coated with an MOF structure to form nano-particles, a TA-PEI shell is formed on the surfaces of the nano-particles, the MOF structure is etched, and finally doxorubicin hydrochloride is absorbed on the surface of the TA-PEI shell. According to the polyphenol nano-reactor, a hollow structure is formed by etching the MOF template, so that the enzyme activity can be maintained; the TA-PEI cross-linked shell is used for protecting enzyme, so that complex environments such as pancreatin can be resisted; and the synergistic treatment of hunger treatment and chemotherapy is realized.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a polyphenol nano-reactor and its preparation method and application. Background technique [0002] Glucose oxidase (GOx) is an exogenous oxidase, which has been widely used in the biomedical field due to its inherent biocompatibility, non-toxicity and efficient and specific catalytic properties for β-D-glucose. GOx can efficiently catalyze the oxidation of glucose to gluconic acid and hydrogen peroxide (H 2 o 2 ), thereby effectively consuming glucose in the tumor area. However, glucose and oxygen, as substrates for GOx-catalyzed reactions, are ubiquitous in the human body. Therefore, when GOx-based nanocarriers circulate in the body, GOx loaded in the nanocarriers will react with glucose and oxygen in the human body to generate H 2 o 2 , H 2 o 2 The production can cause serious systemic adverse reactions. Catalase (CAT) converts H 2 o 2 decomposed into water and...

Claims

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Application Information

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IPC IPC(8): A61K38/44A61K31/704A61K47/34A61K47/22A61P35/00B82Y5/00B82Y40/00
CPCA61K38/443A61K38/44C12Y111/01006A61K31/704A61K47/34A61K47/22B82Y5/00B82Y40/00C12Y101/03004A61P35/00A61K2300/00
Inventor 郭术涛穆菁青杜钰媛李幸薇
Owner NANKAI UNIV
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