Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of EAF1 protein in regulation and control of anthocyanin synthesis and flowering time

A technology of flowering time and anthocyanin, applied in the fields of application, angiosperms/flowering plants, chemical instruments and methods, etc., can solve problems such as unclear molecular basis

Active Publication Date: 2021-10-29
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the molecular basis controlling the specific accumulation of anthocyanins in leaves is still unclear and needs further study

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of EAF1 protein in regulation and control of anthocyanin synthesis and flowering time
  • Application of EAF1 protein in regulation and control of anthocyanin synthesis and flowering time
  • Application of EAF1 protein in regulation and control of anthocyanin synthesis and flowering time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1, the cloning of EAF1 gene

[0096] 1. Phenotype analysis of mutant eaf1

[0097] Take 30 mutant eaf1 seeds or Medicago truncatula R108 seeds, vernalize them at 4°C for 7 days, plant them in nutrient soil, and culture them in alternating light and dark (16h light / 8h dark), observe the phenotype and make statistics.

[0098] See the growth status of some mutants eaf1 and Medicago truncatula R108 when light and dark were alternately cultured for about 40 days figure 1Middle A (WT is Medicago truncatula R108, eaf1 is mutant eaf1). The results showed that the mutant eaf1 had flowered and had grown to 8 nodes on average after 40 days of alternating light and dark culture, while Medicago truncatula R108 had not flowered.

[0099] Count the flowering time and the number of nodes of each strain of Medicago truncatula (that is, the number of days of growth and the number of nodes of Medicago truncatula when the first flower fully opened), and then take the average...

Embodiment 2

[0119] Embodiment 2, acquisition of complementary strains, phenotypic identification and detection of anthocyanin content

[0120] 1. Construction of recombinant plasmid pEAF1pro::EAF1 gDNA

[0121] 1. Using the genomic DNA of Medicago truncatula R108 as a template, using primer EAF1-EcoRI-F: 5′-CCATGATTAC GAATTC GAGTAAAGGCAAACACCACA-3' (the underline is the recognition site of restriction endonuclease EcoRI) and primer EAF1-KpnI-R: 5'-TCGACAGATCCCCG GGTACC A primer pair consisting of TGACGAACCTACCTTTACGT-3' (underlined is the recognition site of restriction endonuclease KpnI) was amplified to obtain a PCR amplification product of about 3250 bp.

[0122] 2. Digest the PCR amplified product obtained in step 1 with restriction endonucleases EcoRI and KpnI, and recover the digested product.

[0123] 3. The pCAMBIA2300 vector was digested with restriction enzymes EcoRI and KpnI, and the vector backbone of about 9850 bp was recovered.

[0124] 4. Ligate the digested product wi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of EAF1 protein in regulation and control of anthocyanin synthesis and flowering time. The amino acid sequence of the EAF1 protein is shown as SEQ ID NO: 2. Experiments prove that a complementary strain is obtained by introducing recombinant plasmid pEAF1pro: EAF1 gDNA into a mutant eaf1; and compared with the mutant eaf1, the complementary strain has the advantages that the content of anthocyanin in leaves is remarkably reduced, the number of flowering sections is increased, and the flowering time is delayed. Therefore, the EAF1 protein can regulate and control anthocyanin synthesis, the number of flowering sections and the flowering time. The EAF1 protein has important application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of EAF1 protein in regulating anthocyanin synthesis and flowering time. Background technique [0002] There are about 700 genera and 18,000 species of legumes (Leguminosae), which are the third largest family after Compositae and Orchidaceae. source, with important economic, ecological and biological value. However, due to factors such as large genomes of most legumes and immature genetic transformation systems, the study of functional genomics of legumes is limited. Medicago truncatula (Medicago truncatula) is considered a model plant for the study of legume genetics because of its small ploidy, small genome, self-pollination, and high number of seeds. Medicago truncatula plant regeneration time is short, there are a large number of mutants and a variety of ecotypes, with high biodiversity. And Medicago truncatula has higher genetic transformation effic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/02A01H5/00A01H6/54
CPCC07K14/415C12N15/825C12N15/827
Inventor 林浩王崇楠牛丽芳
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com