Variable region sequence of specific anti-chlorothalonil antibody and recombinant full-length IgG antibody thereof

Abstract

The invention discloses a variable region sequence of a specific anti-chlorothalonil antibody. The amino acid sequence of a heavy chain variable region coding gene is shown in SEQ ID NO: 2. The invention also discloses an anti-chlorothalonil recombinant full-length IgG antibody. The invention also discloses a recombinant antibody expression plasmid. The heavy chain variable region and light chain variable region sequences of the anti-chlorothalonil recombinant full-length IgG antibody are derived from a monoclonal cell strain which secretes a high-affinity and high-specificity anti-chlorothalonil antibody and is obtained by immunizing mice for multiple times, performing cell fusion and screening. The sequence genes are respectively connected to expression vectors containing a mouse IgG1 heavy chain constant region gene and a mouse kappa light chain constant region gene, the recombinant full-length IgG antibody is obtained by expression of mammalian cells of a double-plasmid system, the recognition activity of the recombinant full-length IgG antibody is very close to that of a mouse-derived parent antibody, large-scale standardized production of the anti-chlorothalonil antibody can be realized, and reliable core raw materials are provided for various immunoassay methods for rapid detection of chlorothalonil residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation and application of a variable region sequence of a specific anti-chlorothalonil antibody and a recombinant full-length IgG antibody. Background technique [0002] Chlorothalonil is a broad-spectrum, protective fungicide that can interact with glyceraldehyde triphosphate dehydrogenase in fungal cells and combine with the cysteine-containing protein in the enzyme to destroy the enzyme activity. The metabolism of the fungal cells is destroyed and the vitality is lost. Chlorothalonil can effectively kill fungi such as Ascomycetes, Deuteromycetes, and Basidiomycetes, but it is highly toxic to non-target organisms, especially fish, and can cause delayed allergic dermatitis in humans. Therefore, the European Commission released The regulations prohibit the use of chlorothalonil and include it in the list of class 2B carcinogens. In addition, wit...

AI Technical Summary

Problems solved by technology

The production cycle of polyclonal antibodies is short, but the specificity is relatively poor; based on hybridoma technology, monoclonal antibodies with high recognition sensitivity and strong specificity can be obtained, but hybridoma cells have complex genetic background and long-term passage and repeated freezing Problems such as gene deletion or mutation that are prone to occur during the resuscitation process cannot ensure the timeliness and stability of different batches of monoclonal antibodies

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