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A metabolomic analysis method for au-pd/wte.coli nanomaterials

A technology of nanomaterials and analysis methods, applied in the field of metabolomics analysis of Au-Pd/wtE.coli nanomaterials, can solve the problem of poor understanding of the interaction between Au-Pd core-shell nanoparticles and organisms, and the inability to determine metabolomics In the analysis method, the biological sample processing conditions, extraction conditions and detection conditions, the internal intervention process of the interaction system, and the molecular mechanism are not clear, so as to avoid abnormal metabolism and uneven sample collection, cost-effective, and simple design.

Active Publication Date: 2022-06-21
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

[0007] At present, there is still little understanding of the interaction between Au-Pd core-shell nanoparticles and organisms, and the internal intervention process and molecular mechanism of the interaction system are still unclear, so it is impossible to determine the metabolomics based on the target metabolites to be detected. Processing conditions, extraction conditions and detection conditions of biological samples in analytical methods

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  • A metabolomic analysis method for au-pd/wte.coli nanomaterials
  • A metabolomic analysis method for au-pd/wte.coli nanomaterials
  • A metabolomic analysis method for au-pd/wte.coli nanomaterials

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Embodiment 1

[0055] Example 1 Non-targeted and spatial metabolomics combined analysis method

[0056] The specific steps of this embodiment are as follows figure 1 shown, including:

[0057] 1. Biological preparation of Au-Pd / wtE.Coli nanomaterials:

[0058] (1) Reagent source

[0059] E. coli wtE.coli (DH5α) strain was purchased from Novagen, Madison, Wisconsin. HUVECs were obtained from the American Tissue Culture Collection (ATCC). Tryptone, Yeast Powder, NaCl, HAuCl 4 ·3H 2 O, K 2 PdCl 4 , HCOONH 4 , 2,2,6,6-tetramethylpiperidine and lutidine-N-oxide were purchased from Sigma-Aldrich (Louis, MO, USA). Dulbecco's modified eagle medium (DMEM), cell culture plates were purchased from Corning (NY, USA). Heat-inactivated fetal bovine serum (FBS), penicillin-streptomycin solution, phosphate buffered saline (PBS) and trypsin were purchased from Gibco (ThermoFisher, USA). 0.22 μm filters were purchased from Biosharp (Anhui, China). All reagents used were of analytical grade, and th...

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Abstract

The invention relates to the fields of nanomaterial preparation and metabolomics analysis, in particular to a metabolomics analysis method for Au-Pd / wtE.coli nanomaterials. The metabolomics analysis method provided by the present invention comprises the following steps: (1) performing DESI-MSI scanning on frozen sections of tissues or organs of animal models to obtain the DESI-MSI detection results of water-soluble metabolites and fat-soluble metabolites; The animal model described above is an animal model given Au-Pd / wtE.coli nanomaterials; wherein, the DESI-MSI detection conditions include: in positive mode, the voltage is 4.5-5kV; in negative mode, the voltage is -4.5-5kV; The spatial resolution is 50-100 μm; the solvent spray composition is 90-95% methanol aqueous solution; (2) spatial metabolomics analysis is performed on the DESI‑MSI detection results. In addition, the metabolomics analysis method of the present invention may also include non-targeted metabolomics detection and analysis of cells co-cultured with Au-Pd / wtE.coli nanomaterials. The invention provides a basis for evaluating Au-Pd / wtE.coli synthesis technology and biological response characteristic evaluation, and has broad application prospects.

Description

technical field [0001] The invention relates to the fields of nanomaterial preparation and metabolomics analysis, in particular to a metabolomics analysis method of Au-Pd / wtE.coli nanomaterials. Background technique [0002] As a modern detection and analysis technology with typical characteristics and significant advantages, metabolomics has attracted great attention of researchers for its intrinsic related principles and practical applications. This technology has shown significant advantages in revealing metabolic processes and life behaviors in vivo. At the same time, research on further technological innovation and development based on modern metabolomics has been ongoing for many years with fruitful results. At present, metabolomics has successfully realized the detection and analysis of the structure and content of thousands of metabolite small molecules (<1500Da), revealing their inherent relationship with organisms. [0003] Due to the advantages of phenotypic b...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64G01N30/02G01N30/72G01N30/86G01N30/88
CPCG01N27/64G01N30/02G01N30/72G01N30/8634G01N30/8696G01N30/88G01N2030/8813G01N2560/00G01N2570/00
Inventor 张定坤龚萌
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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