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A kind of cypress or cypress extract and its application

A technology of extracts and cypress seeds, applied in the field of traditional Chinese medicine extracts, can solve problems such as lack of activity, failure to improve memory retention, etc.

Active Publication Date: 2022-06-28
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Studies have shown that oral administration of 250,500 mg / kg ethanol extract of Cypress seed can improve memory acquisition impairment caused by tonsil damage, but fails to improve memory retention, and has no effect on the activity of choline acetate transferase in cortex, hippocampus and hypothalamus. No effect on activity, no effect on microscopic pathological changes caused by tonsil injury

Method used

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  • A kind of cypress or cypress extract and its application
  • A kind of cypress or cypress extract and its application
  • A kind of cypress or cypress extract and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Preparation of cypress extract

[0076] 1.1 SFC extraction: take 288g of shelled cypress seeds and pulverize them in SFC (SFC instrument model HA221-40-11, Jiangsu Nantong Huaan Supercritical Extraction Co., Ltd.), extraction conditions: extraction kettle temperature: 32°C, extraction kettle temperature and pressure: 25MPa ; Separation kettle temperature: 38°C, separation kettle temperature and pressure: 6MPa; supercritical CO 2 Flow rate; 30-40L / h; extraction time: 1.5h. Deborah seed extract BZ-1-SFC 23.2g, the yield is 8.0wt%.

[0077]1.2 Petroleum ether extraction: Take 5.2 g of shelled cypress seeds and crush them into a 500 ml conical flask, add 250 ml of petroleum ether, and extract by ultrasonic 3 times, 1 hour each time. The petroleum ether extracts were combined and distilled under reduced pressure to obtain 1.2 g of active site BZR-1-PE, with a yield of 23.1 wt%.

Embodiment 2

[0082] Example 2: Determination of the content of helichanoid-type diterpenes araucic acid and 13-epicupressic acid in cypress or cypress seed extract

[0083] 2.1. Separation and purification of helichranoid diterpenes imbricatolic acid and 13-epicupressic acid in cypress seed extract:

[0084] According to the method of literature (Wang YZ. et al., Phytochemistry, 2008, 69, 518-526.), the dried cypress kernels (10.2 kg) were crushed, and then extracted with 95wt% industrial ethanol (20L×3) at room temperature. 81.8 g of the compound imbricatolic acid was obtained by means of column chromatography with a yield of 8.2 wt%; 2.54 g of the compound 13-epicupressic acid was obtained. The spectral data of the compounds araucic acid and 13-epicupressic acid are as follows:

[0085] Araucic acid, imbricatolic acid

[0086] white powder, [α] D (20℃)+50(c 1.5, CHCl3); 1 H-NMR (CDCl 3 ,300MHz):δ0.60(3H,s,CH 3 -20),0.90(3H,d,J=6.4Hz,CH 3 -16),1.22(3H,s,CH 3 -18),3.68(2H,m,H-15),4...

Embodiment 3

[0103] Example 3: Determination of Nootropic Activity of Bozi / Bozi Ren extract series

[0104] Comparison of the improving effects of Baizi / Banziren extract series and marketed health product fish oil on scopolamine-induced memory impairment in mice

[0105] 3.1 Experimental animals

[0106] KM mice, 24-30g for both sexes.

[0107] 3.2 Sample Information

[0108] The SFC extract (BZ-1-SFC), the SFC extract (BZR-SFC), the petroleum ether extract (BZR-PE), and the ethanol extract (BZR-E) were all performed according to Example 1. The method in 2 was obtained from the extraction of cypress seeds; fish oil was purchased from General Nutrition Corporation, GNC, USA.

[0109] 3.3 Experimental equipment

[0110] Channel type mouse water maze (80cm×50cm×20cm)

[0111] 3.4 Sample processing

[0112] 1) Compounds were dissolved in a 20 wt% glycerol aqueous solution. Except for BZR-E, which was measured at a concentration of 20 mg / kg, the other five compounds were all measured at a...

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Abstract

The invention provides the application of cypress seed or cypress seed extract. Specifically, the Fructus Cypress or Fructus Cypress extract of the present invention is used to have good anti-fatigue effect, and can be used to prepare an anti-fatigue composition, wherein, the Fructus Cypress extract has one or more of the following characteristics: (a) the extract The molecular weight distribution of the extract is 280-310; (b) the total content of unsaturated fatty acids in the extract ≥ 80wt%; and / or (c) the total content of diterpenoids derived from Cypress seeds in the extract ≤ 0.5wt %. The cypress seed or the cypress seed extract of the present invention has wide application in the fields of food and health products.

Description

[0001] This application is a divisional application of an invention application with an application date of August 12, 2013, an application number of 201310349888.5, and an invention title of "A kind of cypress or cypress extract and its use". technical field [0002] The present invention relates to the field of traditional Chinese medicine extracts, in particular, to a high active ingredient extracted from cypress radix, which is used as a drug for improving cognitive ability, improving cognitive dysfunction, anti-fatigue and functional food. Background technique [0003] Cognitive function refers to the ability of the human brain to process, store and retrieve information, including memory, language, visual space, execution, calculation, and understanding and judgment. [1] . Cognitive dysfunction refers to the impairment of one or more of the above-mentioned abilities. It is common in patients with various types of dementia, of which the highest incidence is Alzheimer's d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/14A61P39/00A61P25/28A23L33/105A61K131/00A23L33/00
CPCA23L33/105A61K36/14A23V2002/00A61K2236/35A61K2236/37A61K2236/39A23V2200/30A23V2250/21
Inventor 叶阳唐希灿章海燕姚胜王维季萍
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI