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Cedar seed or cedar seed extract and application thereof

A technology of extracts and cypress seeds, applied in the field of traditional Chinese medicine extracts, can solve problems such as lack of activity, failure to improve memory retention, etc.

Active Publication Date: 2021-11-05
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Studies have shown that oral administration of 250,500 mg / kg ethanol extract of Cypress seed can improve memory acquisition impairment caused by tonsil damage, but fails to improve memory retention, and has no effect on the activity of choline acetate transferase in cortex, hippocampus and hypothalamus. No effect on activity, no effect on microscopic pathological changes caused by tonsil injury

Method used

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  • Cedar seed or cedar seed extract and application thereof
  • Cedar seed or cedar seed extract and application thereof
  • Cedar seed or cedar seed extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Embodiment 1: the preparation of cypress seed extract

[0076] 1.1 SFC extraction: Take 288g of Cypress with shell and crush it into SFC (SFC instrument model HA221-40-11, Jiangsu Nantong Huaan Supercritical Extraction Co., Ltd.), extraction conditions: extraction kettle temperature: 32°C, extraction kettle temperature and pressure: 25MPa ;Separation tank temperature: 38°C, separation tank temperature and pressure: 6MPa; supercritical CO 2 Flow rate; 30-40L / h; extraction time: 1.5h. Obtain 23.2 g of the extract BZ-1-SFC of Cypress seed, and the yield is 8.0 wt%.

[0077]1.2 Petroleum ether extraction: take 5.2 g of cypress seeds without shell and grind them into a 500ml Erlenmeyer flask, add 250ml of petroleum ether, and perform ultrasonic extraction 3 times, 1 hour each time. The petroleum ether extracts were combined and distilled under reduced pressure to obtain 1.2 g of the active part BZR-1-PE, with a yield of 23.1 wt%.

Embodiment 2

[0082] Embodiment 2: Determination of the content of the helicanane type diterpene araucine acid and 13-epicupressic acid (13-epicupressic acid) in Cypress seed or Cypress kernel extract

[0083] 2.1. Separation and purification of the helicanoid diterpenes imbricatolic acid and 13-epicupressic acid in the extract of Cypress kernels:

[0084] According to the method of literature (Wang YZ.et al., Phytochemistry, 2008,69,518-526.), after the dried cypress kernel (10.2kg) was crushed, it was extracted with 95wt% industrial ethanol (20L×3) at room temperature, and after different 81.8 g of the compound imbricatolic acid (imbricatolic acid) was obtained by means of column chromatography, and the yield was 8.2 wt %; 2.54 g of the compound 13-epicupressic acid was obtained. The spectral data of the compound araucine acid and 13-epicupressic acid are as follows:

[0085] Araucine acid, imbricatolic acid

[0086] white powder, [α] D (20°C)+50(c 1.5, CHCl3); 1 H-NMR (CDCl 3 ,300MH...

Embodiment 3

[0103] Example 3: Determination of Nootropic Activity of Baizi / Baiziren Extract Series

[0104] Comparison of the improvement effect of cypress seed / both kernel extract series and marketed health product fish oil on scopolamine-induced memory impairment in mice

[0105] 3.1 Experimental animals

[0106] KM mice, 24-30g male and female.

[0107] 3.2 Sample information

[0108] Baizi SFC extract (BZ-1-SFC), Baiziren SFC extract (BZR-SFC), Baiziren petroleum ether extract (BZR-PE) and Baiziren ethanol extract (BZR-E) are all according to Example 1 The method in the method is extracted from Baiziren; fish oil is purchased from the United States General Nutrition Corporation, GNC.

[0109] 3.3 Experimental equipment

[0110] Channel mouse water maze (80cm×50cm×20cm)

[0111] 3.4 Sample processing

[0112] 1) The compounds were dissolved in 20 wt% glycerol aqueous solution. Except for BZR-E whose measured concentration was 20 mg / kg, the measured concentrations of the other 5 c...

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Abstract

The invention provides application of cedar seed or a cedar seed extract. Specifically, the cedar seed or the cedar seed extract has a good anti-fatigue effect and can be used for preparing an anti-fatigue composition, and the cedar seed extract has one or more of the following characteristics: (a) the molecular weight distribution of the extract is 280-310; (b) the total content of unsaturated fatty acid in the extract is greater than or equal to 80wt%; and / or (c) the total content of diterpenoid substances derived from cedar seeds in the extract is less than or equal to 0.5wt%. The cedar seed or the cedar seed extract has wide application in the fields of food and health care products.

Description

[0001] This application is a divisional application of an invention application with a filing date of August 12, 2013, an application number of 201310349888.5, and an invention title of "A Cypress Semen or Cypress Semen Extract and Its Use". technical field [0002] The invention relates to the field of extracts of traditional Chinese medicines, in particular to a high active ingredient extracted from cypress seeds, which can be used to improve cognitive ability, improve cognitive dysfunction, anti-fatigue medicine and functional food. Background technique [0003] Cognitive function refers to the ability of the human brain to process, store and extract information, including memory, language, visual space, execution, calculation and understanding and judgment. [1] . Cognitive dysfunction refers to the impairment of one or more of the above abilities, which is common in various types of dementia patients, among which the most common are Alzheimer's disease (Alzheimer's disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/14A61P39/00A61P25/28A23L33/105A61K131/00A23L33/00
CPCA23L33/105A61K36/14A23V2002/00A61K2236/35A61K2236/37A61K2236/39A23V2200/30A23V2250/21
Inventor 叶阳唐希灿章海燕姚胜王维季萍
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI