Cultivation method for improving emergence rate of extremely-early-maturing pearl pear filial generation
A method for cultivating early-maturing pears and a technology for early-maturing pears are applied in the field of cultivation for improving the emergence rate of hybrid progeny of extremely early-maturing pears and pearl pears, and can solve the problems of incomplete development of seed embryos, difficulty in obtaining seedlings, restricting the utilization of pearl pears, and the like, and achieve robust growth of seedlings. , to achieve the effect of variety selection and shortening the breeding cycle
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Embodiment 1
[0040] Embodiment 1, the impact of different low temperature treatment time on germination of seed embryo
[0041] After sterilization, the seeds were transferred to conventional plant MS medium, and placed at 4°C for low-temperature cultivation. After 0, 25, 50, 75, 100, and 125 days of low temperature treatment, the seeds were taken out in batches, placed in a light culture room at 28°C for cultivation, and the germination of the seeds was observed after 12 days.
[0042] The hybrid fruit seeds were taken out and sterilized, sown on plant MS medium, treated at a low temperature of 4°C, taken out at 0, 25, 50, 75, 100, and 125 days respectively, and placed in a light culture room at 28°C for cultivation. Seed germination was observed on the 12th day after taking it out. The results showed that with the increasing of low temperature treatment time, the germination rate of seeds in the medium was also increasing. After low temperature treatment for 100 days, the germination r...
Embodiment 2
[0043] Embodiment 2, the impact of different seed cold storage methods on germination of seed embryos
[0044] Mature fruits were stored in a cold storage at 4°C for 0, 25, 50, 75, 100, and 125 days, and then the fruits were taken out in batches, and the seeds were sterilized and transferred to MS culture. They were cultured in a light culture room at 28°C, and the fruits were observed at different times after 12 days. Seed germination after preservation.
[0045] In addition, for pearl pears with fruits stored in a cold storage at 4°C, the fruits were cut open at 0, 25, 50, 75, 100, and 125 days to take out the seeds. The plump seeds were sterilized and sown on plant MS medium, and placed at 28°C for light cultivation. Observing the germination of the seeds after the 12th day of cultivation, it was found that the overall germination rate of the seeds after fruit preservation was low after low temperature treatment, and the highest germination rate was only 6.9% after 100 day...
Embodiment 3
[0046] Embodiment 3, the impact of different seed coat breaking modes on seed embryo germination
[0047] Pearl pear seeds preserved with fruit after low-temperature treatment for 100 days were subjected to skin-breaking treatment. The treatment methods were tip cutting (removing the seed coat near the radicle), middle cutting (removing the upper 1 / 2 of the cotyledons), and lateral cutting (removing the seed coat on the side of the seed). ) and full peeling (all the seed coats were removed), and the untreated seeds were used as controls to observe the germination of seed embryos after different cutting methods.
[0048] The seeds of offspring pear pears with fruit cryopreserved for 100 days were subjected to different seed coat removal treatments, and the germination of the seeds was observed at 6, 12 and 18 days after incision. The results are shown in the figure, on the 18th day after the seed coat was broken, the seed germination rate of each treatment reached the maximum v...
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