Construction method and application of high performance liquid chromatography fingerprint spectrum of Zilongjin tablets
A high-performance liquid chromatography and fingerprint technology, which is applied in the field of traditional Chinese medicine identification, can solve problems such as inability to cover detection, and achieve the effect of ensuring safety and effectiveness and ensuring clinical efficacy.
Active Publication Date: 2021-11-12
LONGSHUNRONG PHARMA FACTORY TIANJIN ZHONGXIN PHARMA GRP +1
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Problems solved by technology
There are only three identifications and one content determination in the pharmacopoeia standards, which cannot cover the detection of many ingredients in the eight herbs in Zilongjin Tablets
Method used
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Embodiment 1
[0144] Instruments and reagents:
[0145] Instrument: SHIMADAZU LC-20AD liquid chromatograph, including autosampler and diode array detector; LCSolution workstation.
[0146] Column: Waters Symmetry C 18 (4.6×250mm, 5μm) chromatographic column;
[0147] phenomenex Luna C 18 (2) (250×4.6mm, 5μm) chromatographic column;
[0148] ACCHROM XAqua C 18 (4.6×250mm, 5μm) chromatographic column;
[0149] SHEISHIDO CAPCELL C 18 (4.6×250mm, 5μm) chromatographic column;
[0150] Agilent ZORBAX SB-C 18 (250×4.6mm, 5μm) chromatographic column.
[0151] Reagents: acetonitrile, methanol, glacial acetic acid (chromatographically pure, Tianjin Concord Technology Co., Ltd.); phosphoric acid, potassium dihydrogen phosphate (analytical pure, Tianjin Yingda Rare Chemical Reagent Factory); chloroform, ethyl acetate 1. Hydrochloric acid (analytically pure, Tianjin Concord Technology Co., Ltd.) trifluoroacetic acid (chromatographically pure, TEDIA); water is deionized water.
[0152] Referenc...
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The invention provides a construction method and application of a high performance liquid chromatography fingerprint spectrum of a Zilongjin tablets, and the method comprises the following steps: preparation of a test solution, preparation of a corresponding reference solution and a corresponding reference medicinal material solution, construction of a reference fingerprint spectrum and a fingerprint spectrum of the test solution, determination of characteristic peaks of different medicinal materials contained in the Zilongjin tablets, and verification of characteristic spectrum methodology. According to the invention, analysis conditions such as optimal mobile phase composition, gradient elution procedure, chromatographic column, detection wavelength, detection column temperature, detection flow velocity and the like are screened out through a large number of experiments. Through verification of characteristic chromatogram methodology, the method provided by the invention can be used for comprehensively, objectively and accurately detecting and evaluating the medicine quality of the Zilongjin tablets, the safety and effectiveness of the Zilongjin tablets can be guaranteed from the source, and the clinical curative effect is guaranteed.
Description
technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicines, in particular to a construction method and application of a high performance liquid chromatography fingerprint of Zilongjin Tablets. Background technique [0002] Zilongjin Tablet is an exclusive variety produced by Tianjin Zhongxin Pharmaceutical Group Co., Ltd. Longshunrong Pharmaceutical Factory. The quality standard is recorded in the 2010 edition of "Chinese Pharmacopoeia". There are only three identifications and one content determination in the Pharmacopoeia standard, which cannot cover the detection of many components in the eight herbs in Zilongjin Tablets. [0003] In view of this, the present invention is proposed. Contents of the invention [0004] One of the purposes of the present invention is to provide a method for constructing high performance liquid chromatography fingerprints of Zilongjin Tablets, which can comprehensively, objectively...
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IPC IPC(8): G01N30/06G01N30/74G01N30/86
CPCG01N30/06G01N30/74G01N30/8686Y02A50/30
Inventor 周建宇李萍周军赵晨刘伟陈云霞侯建松
Owner LONGSHUNRONG PHARMA FACTORY TIANJIN ZHONGXIN PHARMA GRP