Unlock instant, AI-driven research and patent intelligence for your innovation.

Mongolian culter sperm cryopreservation technology

A technology for cryopreservation and sperm, which is applied in the cryopreservation of biological materials/tissues, and in the biological field. It can solve the problems of no sperm cryopreservation scheme of C. mongolicus, and achieve the effect of easy recovery, good preservation effect, and rapid thawing process.

Pending Publication Date: 2021-11-16
SHANGHAI OCEAN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no cryopreservation solution specifically for the sperm of Cichlid mongolicus. The closest solution is the method of freezing the sperm of Cichlid mongolicus. Therefore, in order to realize the cryopreservation of the sperm of Cichlid mongolicus, it is necessary to develop a new storage solution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mongolian culter sperm cryopreservation technology
  • Mongolian culter sperm cryopreservation technology
  • Mongolian culter sperm cryopreservation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of diluent D-15: The formula of this diluent is 8g / L NaCl, 0.5g / L KCl, 15g / L glucose. The specific preparation process is: weigh 1.6g NaCl, 0.1g KCl, 3.0 g of glucose, put it into a 500mL beaker, add about 150mL of ultrapure water; transfer to a 200mL volumetric flask, and set the volume to 200mL; pour it into a 250mL suction filter bottle, filter; store at 4°C.

[0028] (2) Preparation of antifreeze solution: Measure 36mL of D-15 dilution with a graduated cylinder and pour it into a 50mL centrifuge tube; absorb 4mL of ethylene glycol with a 5mL pipette gun and add it to the above centrifuge tube; weigh 0.2g with an analytical balance Add trehalose to the above centrifuge tube; mix thoroughly with a vortex shaker.

[0029] (3) Sperm collection: During the breeding season of Culturus mongolicus, cast a net to fish out the males of Culturus mongolicus, select sexually mature healthy males, dry around the cloaca with a dry towel, gently press the abdomen, a...

Embodiment 2

[0033] (1) Preparation of diluent D-15: The formula of this diluent is 8g / L NaCl, 0.5g / L KCl, 15g / L glucose. The specific preparation process is: weigh 1.6g NaCl, 0.1g KCl, 3.0 g of glucose, put it into a 500mL beaker, add about 150mL of ultrapure water; transfer to a 200mL volumetric flask, and set the volume to 200mL; pour it into a 250mL suction filter bottle, filter; store at 4°C.

[0034] (2) Preparation of antifreeze solution: Measure 36mL of D-15 dilution with a graduated cylinder and pour it into a 50mL centrifuge tube; absorb 4mL of ethylene glycol with a 5mL pipette gun and add it to the above centrifuge tube; weigh 0.2g with an analytical balance Add trehalose to the above centrifuge tube; mix thoroughly with a vortex shaker.

[0035](3) Sperm collection: During the breeding season of Culturus mongolicus, cast a net to fish out the males of Culturus mongolicus, select sexually mature healthy males, dry around the cloaca with a dry towel, gently press the abdomen, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Mongolian culter sperm cryopreservation technology which is characterized by comprising the steps of diluent preparation, anti-freezing liquid preparation, sperm collection, fresh sperm activity detection, sperm subpackaging, cooling, heating unfreezing and frozen sperm activity detection. The compound formula of ethylene glycol and trehalose is adopted, a better preservation effect is achieved, the Mongolian culter sperms are recovered after being cryopreserved for one month, and the activation rate reaches 70% or above. According to the invention, the adopted freezing container is a 0.25 mL straw, and the amount of sperms contained in each straw is only about 0.03 mL actually. A small-quantity and multi-tube mode is adopted, and the number of resuscitation / unfreezing tubes can be flexibly selected during resuscitation verification and unfreezing activation in the later period. The unfreezing process is rapid, operation is simple, and mass sperms are easy to resuscitate.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of cryopreservation of biological materials / tissues, especially sperm cryopreservation technology. Background technique [0002] Since the cryopreservation technology of biological gametes first succeeded in the 1950s, after decades of development, it is currently playing an important role in the field of human medicine, the preservation of excellent genetic breeding of animals and plants, and the germplasm preservation of wild endangered species. role. Among them, there are relatively few studies on the cryopreservation of fish sperm, but there are also some fish sperm that have been cryopreserved, such as Otaki six line fish (patent number CN202110469108.5), spring fish (patent number CN202110227761.0), Brown-spotted grouper (patent number CN201710039157.9), etc., and most economic fish in my country have not yet achieved sperm cryopreservation. Mongolian cypriniformes b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 张俊芳韩兵社蔡心清朱中秋谢婷婷张全根张杰
Owner SHANGHAI OCEAN UNIV