Tumor whole-cell targeting reagent as well as preparation method and application thereof
A targeting and reagent technology, applied in the field of immunology, can solve the problems of inability to realize large-scale mass production of tumor whole cell vaccines in vitro, difficulty in preparing tumor whole cell vaccines, and few autologous tumor cells, so as to reduce the risk of occurrence and prepare The method is simple and easy to implement, and the effect of biocompatibility is good
Pending Publication Date: 2021-11-16
SUN YAT SEN UNIV
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Problems solved by technology
However, in practical applications, in the early stage of cancer, there are few autologous tumor cells, which are difficult to obtain, and are difficult to be used in the preparation of tumor whole cell vaccines, and there are certain risks in using allogeneic cultured tumor cells to prepare antigens. Therefore, it is impossible to achieve In vitro mass production of tumor whole cell vaccines
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preparation example Construction
[0081] Preparation of tumor whole cell targeting reagent (vaccine)
[0082] Connect a fluorescent group FAM to the 3' end of the nucleic acid aptamer AS1411, and then chemically modify a 2-imidazole formaldehyde at the 5' end of AS1411 connected to the fluorescent group FAM (the specific steps are: first connect the fluorescent group The 5' end of FAM's AS1411 is connected to an NH 2 -C6 group, 2-imidazole formaldehyde and NH at the 5' end of AS1411 by aldehyde-amine condensation 2 -C6 group connection), so that the 5' end of AS1411 has the ligand group required for the synthesis of ZIF-8.
[0083] The specific preparation method is:
[0084] Dissolve 2 μM AS1411 and 100 μM 2-imidazole formaldehyde in PBS buffer solution at pH 6.8, place at 37°C for 24 hours at a constant temperature and avoid light, and shake slowly to obtain the tumor whole cell targeting reagent (AS1411-1).
[0085] Synthesis flow chart as figure 2 shown.
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Abstract
The invention discloses a tumor whole-cell targeting reagent as well as a preparation method and application thereof, and the tumor whole-cell targeting reagent contains nucleic acid molecules with a cancer cell membrane targeting killing effect. The nucleic acid molecule is composed of a cancer cell membrane targeting sequence and a metal organic framework ligand connected to the 5' end of the cancer cell membrane targeting sequence. The cancer cell membrane targeting sequence disclosed by the invention is wide in application range, can accurately coat autologous or allogenic tumor cells, is not limited to a single cancer species, and is effective to cancer cells of a wide variety of cancer species. Moreover, the hydrogel is good in biocompatibility, high in safety and free of killing effect on normal cells, the preparation method is simple and easy to implement, the steps of ultraviolet / repeated freeze thawing and the like are not needed, and the hydrogel can react at normal temperature.
Description
technical field [0001] The invention belongs to the field of immunology, and in particular relates to a tumor whole cell targeting agent and its preparation method and application. Background technique [0002] Cancer is one of the major diseases that seriously threaten human health. In related technologies, the main treatment strategies for cancer include surgery, chemotherapy, radiotherapy, etc. Although these treatment methods can inhibit tumor growth in the short term, they have no good inhibitory effect on cancer recurrence and metastasis, and the long-term survival rate of patients is still worrying. Currently, there are no clinically effective management methods for cancer metastasis or recurrence, and effective cancer treatment is fundamentally difficult due to the existence of cancer heterogeneity. [0003] Cancer immunotherapy is a treatment method that uses the patient's own immune system to identify, attack, and eliminate tumor cells and prevent recurrence. Ther...
Claims
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IPC IPC(8): A61K39/00A61K47/69A61K39/39A61P35/00A61P35/04A61P37/04A61K49/00C12N15/11
CPCA61K39/0011A61K47/6949A61K39/39A61P35/00A61P35/04A61P37/04A61K49/0043A61K49/0052C12N15/11A61K2039/55511A61K2039/575
Inventor 戴宗杨慧慧柳思扬邹小勇
Owner SUN YAT SEN UNIV