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Catalytically luminous copper cluster nano-enzyme as well as preparation method and application thereof

A nano-enzyme and copper cluster technology, applied in the fields of catalysis and biological nanomaterials, can solve the problems of complex equipment and cumbersome methods, and achieve the effects of high sensitivity, mild preparation method and good accuracy

Active Publication Date: 2021-11-30
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some of the disclosed methods require complex equipment, and some methods are cumbersome. It is very important to develop simple, fast and highly sensitive adrenaline detection methods

Method used

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  • Catalytically luminous copper cluster nano-enzyme as well as preparation method and application thereof
  • Catalytically luminous copper cluster nano-enzyme as well as preparation method and application thereof
  • Catalytically luminous copper cluster nano-enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of cysteine-histidine-copper nanocluster Cys-His-CuNCs

[0035] Add 1 mL of 30 mM cysteine ​​(Cys) aqueous solution to 1 mL of 10 mM histidine (His) aqueous solution under magnetic stirring, add 10 μL of 50 mM Cu(NO 3 ) 2 The aqueous solution, stirred evenly, constitutes Cys:His:Cu 2+ The molar ratio of the mixture is 3:1:0.05. Slowly add 0.5M NaOH solution to the mixture to keep the pH value of the mixture at 8-9. When the solution gradually turns yellow (about 30min), centrifuge the mixture at 10 000rpm×10min to collect clear light yellow The supernatant was used as the prepared copper nanocluster (Cys-His-CuNCs) solution. The concentration of copper nanoclusters was calculated based on the concentration of added copper ions to be 0.25 mM.

[0036] figure 1 is a transmission electron microscope picture of the prepared Cys-His-CuNCs, showing that the Cys-His-CuNCs have a particle size of about 2nm. figure 2 is the fluorescence spectrum (cur...

Embodiment 2

[0037] Example 2 Preparation of cysteine-histidine-copper nanocluster Cys-His-CuNCs

[0038] Add 1 mL of 50 mM cysteine ​​(Cys) aqueous solution to 1 mL of 10 mM histidine (His) aqueous solution under magnetic stirring, add 10 μL of 50 mM Cu(NO 3 ) 2 The aqueous solution, stirred evenly, constitutes Cys:His:Cu 2+ The molar ratio of the mixture is 5:1:0.05. Slowly add 0.5M NaOH solution to the mixture to keep the pH value of the mixture at 8-9. When the solution gradually turns yellow (about 30min), centrifuge the mixture at 10 000rpm×10min to collect clear light yellow The supernatant was used as the prepared copper nanocluster (Cys-His-CuNCs) solution. figure 2 is the fluorescence spectrum (curve 1) of the prepared Cys-His-CuNCs, and the emission peak of Cys-His-CuNCs is at 480nm.

Embodiment 3

[0039] Example 3 Preparation of cysteine-histidine-copper nanocluster Cys-His-CuNCs

[0040] Add 1 mL of 10 mM cysteine ​​(Cys) aqueous solution to 1 mL of 10 mM histidine (His) aqueous solution under magnetic stirring, add 10 μL of 50 mM Cu(NO 3) 2 The aqueous solution, stirred evenly, constitutes Cys:His:Cu 2+ The molar ratio of the mixture is 1:1:0.05. Slowly add 0.5M NaOH solution to the mixture to keep the pH value of the mixture at 8-9. When the solution gradually turns yellow (about 30min), centrifuge the mixture at 10 000rpm×10min to collect clear light yellow The supernatant was used as the prepared copper nanocluster (Cys-His-CuNCs) solution. figure 2 is the fluorescence spectrum (curve 3) of the prepared Cys-His-CuNCs, and the emission peak of Cys-His-CuNCs is at 480nm.

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Abstract

The invention discloses a catalytically luminous copper cluster nano-enzyme as well as a preparation method and application thereof in catalysis and determination of epinephrine. The catalytically luminous copper cluster nano-enzyme is a copper nano-cluster formed by reaction of copper ions, cysteine and histidine. The prepared copper nano-cluster not only has the activity of natural laccase, can catalyze the reaction of epinephrine like laccase, but also has a luminous function, and can emit light to indicate epinephrine molecules at nM level. The preparation method of the copper cluster nano-enzyme is simple and convenient, and the copper cluster nano-enzyme has a good application prospect in the aspects of catalysis, sensing, biodegradation and the like.

Description

technical field [0001] The invention relates to a copper cluster nanozyme that catalyzes luminescence, a preparation method thereof, and an application in luminescent detection of adrenaline. The prepared copper cluster nanozyme can catalyze degradation and sense biomolecules, and belongs to the field of catalysis and biological nanometer materials. Background technique [0002] Metal nanoclusters composed of several to hundreds of atoms not only have unique optical, electrical, magnetic, and catalytic properties, but also have ultra-small size and good biocompatibility. There are a wide range of potential applications in other fields. Reports on the use of copper nanoclusters for catalysis include: Chinese Patent Publication No. CN111521589A, 2019, Wei Shoulian et al., a method for preparing fluorescent copper nanoclusters-carbon composite catalysts, which discloses a method using cysteine ​​as a template and reducing agent , a preparation method for preparing copper nanoc...

Claims

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Application Information

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IPC IPC(8): B01J31/22C09K11/06B82Y20/00B82Y40/00G01N21/64
CPCB01J31/2243B01J31/223B01J31/003C09K11/06G01N21/6428B82Y20/00B82Y40/00B01J2531/16C09K2211/188
Inventor 陈扬王莉
Owner SOUTHEAST UNIV
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