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Bacillus velezensis and application thereof in preventing and treating various vegetable diseases

A technology of bacillus and plant diseases, applied in the direction of application, bacteria, fungicides, etc., can solve problems such as drug residues, environmental pollution, ecological imbalance, etc.

Active Publication Date: 2021-11-30
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Plant diseases are usually controlled by chemical agents, but the large-scale use of chemical agents can easily cause problems such as environmental pollution, ecological imbalance, drug residues, food safety, etc. Therefore, low-cost, high-efficiency, environmentally friendly and drug-free biological methods are used to prevent Controlling plant diseases has gradually become a hot spot at home and abroad

Method used

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  • Bacillus velezensis and application thereof in preventing and treating various vegetable diseases
  • Bacillus velezensis and application thereof in preventing and treating various vegetable diseases
  • Bacillus velezensis and application thereof in preventing and treating various vegetable diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. Isolation, screening, identification and preservation of bacterial strain ZF438

[0063] 1. Isolation and purification of strain ZF438

[0064] Soil samples were collected from the capsicum rhizosphere in the open field in Shouguang, Shandong, and 10 g of each soil sample was weighed, added to 90 mL of sterilized water, placed in a constant temperature shaker at 28 °C for 30 min, and then treated in a water bath at 80 °C for 30 min. according to 10 -1 -10 -7 For serial dilution, take a dilution gradient of 10 -5 、10 -6 、10 -7 The soil samples were plated and placed in a constant temperature incubator at 28°C for 24 hours. After a single colony grows on the coated plate, use a sterilized toothpick to pick out a single colony of different shapes and line it on the LB solid plate for purification. Place it in a constant temperature incubator at 28°C until a single colony with the same shape grows, and then place it at 4°C. Store in the refrigerator for lat...

Embodiment 2

[0091] Embodiment 2, Veles bacillus ZF438 antibacterial spectrum determination

[0092] Eight common vegetable pathogenic fungi and nine pathogenic bacteria were selected to determine the antibacterial spectrum of Bacillus Velez ZF438.

[0093] The specific sources of the eight vegetable pathogenic fungi are as follows:

[0094] Colletotrichum capsici (Syd.) E.J.Butler&Bisby has been published in the literature "Du Gongfu, Qi Zhiqiang, Li Baoju, Guo Yinglan, Yang Yan. Research on the diversity of asexual fungi in winter vegetables in Hainan. Fungal Research 2016,14(03):142- 148.", the public can obtain it from the Vegetable and Flower Research Institute of the applicant Chinese Academy of Agricultural Sciences to repeat the present invention, and cannot be used for other purposes.

[0095] Botrytis cinerea has been published in the literature "Shi Yanxia, ​​Tang Ming, Jin Zhiwen, Xie Xuewen, Chai Ali, Li Baoju. Evaluation of the resistance of Botrytis cinerea to different typ...

Embodiment 3

[0120] Embodiment 3, Bacillus Velez ZF438 growth and pH variation curve determination

[0121] Pick a single colony of Bacillus Velez ZF438 in LB liquid medium, shake it at 28°C and 180r / min for 16 hours, then use blank LB liquid medium to adjust the concentration of the bacterial suspension to OD 600nm 0.6, inoculated in LB liquid medium (100mL / 250mL) with 2% inoculum size. 28°C, 180r / min shaking culture for 48h, measure OD every 4h 600nm value and pH.

[0122] The result is as Figure 5 As shown, the results show that: Bacillus Velez ZF438 grows faster in LB liquid medium, directly enters the logarithmic growth phase after seed liquid inoculation, and enters the stationary phase after 20h, OD 600nm The value is stable around 1.83. The pH increased continuously with the growth of the strain ZF438, and was 8.07 after 48 hours.

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Abstract

The invention discloses bacillus velezensis and an application thereof in preventing and treating various vegetable diseases. The bacillus velezensis with strain number of ZF438 is preserved in the China General Microbiological Culture Collection Center with registration number of CGMCC No.22291. Experiments prove that the bacillus velezensis ZF438 has an inhibition effect on various pathogenic fungi and bacteria, and has a relatively good inhibition effect on the pathogenic fungi such as rhizoctonia solani, colletotrichum capsici and stemphylium lycopersici. Through in-vitro and potting control effect determination on anthracnose of capsicum fruits, the control effect of the bacillus velezensis ZF438 on the colletotrichum capsici is obviously higher than that of a control group. Meanwhile, the control effect on cucumber soft rot disease and the potting control effect on tomato bacterial wilt are both higher than a control group. In actual production, the bacillus velezensis ZF438 can be widely applied to prevention and treatment of various vegetable diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of bacillus veles and its application in preventing and treating various vegetable diseases. Background technique [0002] Plant diseases are usually controlled by chemical agents, but the large-scale use of chemical agents can easily cause problems such as environmental pollution, ecological imbalance, drug residues, food safety, etc. Therefore, low-cost, high-efficiency, environmentally friendly and drug-free biological methods are used to prevent The control of plant diseases has gradually become a hot spot at home and abroad. In recent years, countries have strengthened the supervision of environmental protection and put forward higher requirements for the sustainable development of agriculture. Biological control has become a research hotspot in plant disease control due to its unique advantages (Zhou Tong, Xu Yongping, Wang Lili, Chen Yan , Zhang Nan, Wang...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00C12R1/07
CPCC12N1/20A01N63/22Y02A40/10
Inventor 李磊李宝聚黄艺烁赵昱榕谢学文石延霞柴阿丽
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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