Vaccine immunologic adjuvant and application thereof in vaccine preparation
An immune adjuvant and vaccine technology, applied in the field of vaccine immune adjuvants and its application in the preparation of vaccines, can solve the problems of complex components, biological safety to be verified, etc., and achieve the effect of promoting immune function
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Embodiment 1
[0063] In this embodiment, it is used to collect PBMC cell samples of responders and non-responders among volunteers vaccinated with the new crown inactivated vaccine.
[0064] (1) Recruiting research vaccinators:
[0065] Inclusion criteria: 18-50 years old, gender is not limited, the sample size is sufficient;
[0066] Exclusion criteria: family and personal history of convulsions, history of epilepsy, allergies, whether to inject immunoglobulin at least one month apart. All enrolled volunteers were excluded from the new coronavirus infection and heart, brain, kidney and other organ diseases.
[0067] (2) Collect samples
[0068] Time for sample collection: Recruit volunteers who received the new crown inactivated vaccine, receive 2 doses of vaccine according to regulations, and the interval between the two doses of vaccine is 28 days, collect serum and cells before immunization, 28 days after the first immunization, and one month after the second immunization.
[0069] E...
Embodiment 2
[0075] In this embodiment, the expression of cytokine co-stimulatory molecules in PBMC was analyzed by real-time PCR.
[0076] (1) Experimental grouping:
[0077] Group A: response group, vaccine responder PBMC + new crown S protein or RBD protein;
[0078] Group B: GL+S / RBD+ non-response group, vaccine non-responders PBMC+ new crown S or RBD protein+GL;
[0079] Group C: S / RBD+ non-response group, vaccine non-responder PBMC+ new crown S or RBD protein;
[0080] Group D: GL+non-response group, PBMC+GL of vaccine non-responders.
[0081] The specific grouping situation is shown in Table 1 below:
[0082] Table 1
[0083]
[0084] (2) Real-time RCR analysis of PBMC cytokine co-stimulatory molecular method
[0085] The above four groups, groups A, B and C were all stimulated with S or RBD at a final concentration of 5 μg / mL, the GL+S / RBD stimulation group was also stimulated with GL with a final concentration of 10 μg / mL, and group D was only given a final concentration o...
Embodiment 3
[0093] This example is used to analyze the proliferative response and CTL killing activity of GL to allogeneic lymphocytes in non-responders to the new crown vaccine.
[0094] (1) Induction of DC
[0095] Take the PBMC of the research object (the concentration is 2×10 6 mL -1 ), added to a 6-well plate (2mL / well), 37°C, 5% CO2 After 2 hours of incubation in the incubator, adherent cells and suspension cells were collected respectively.
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