The present invention provides a portable wood floor grooving machine which is characterized by comprising a milling cutter device, a feeding device, a clamping positioning device and a frame. The milling cutter device and the feeding device are installed on the frame. The clamping positioning device is installed on the feeding device. The milling cutter of milling cutter device rotates in a horizontal plane. The clamping positioning device executes linear reciprocating motion under the driving of feeding device. The clamping feeding device is mainly composed of an eccentric wheel, an eccentric wheel handle, a tension rod shaft, a tension rod, a supporting plate, an upper splint, a lower splint, a spring and a guiding lever. The feeding device is mainly composed of a feeding motor, a feeding motor support, a coupling, a front support of guide screw, a guide screw, a feed screw nut, a straight slide way, a sliding block and a back support of guide screw. The milling cutter device is mainly composed of the milling cutter, a support, a cutter base, a base frame, a milling cutter motor, a regulating knob, an ejector rod, a regulating wheel and a clamping sheet. The wood floor grooving machine of the invention has the advantages of light weight, excellent portability, simple structure, convenient operation and low fabricating cost.