Tianshan chlorella and Multi-stage cyclic extraction equipment and preparation method of chlorella deep purification factors

A technology for purifying factors and chlorella, applied in chemical instruments and methods, cosmetic preparations, solid solvent extraction, etc., can solve the problems of insufficient contact between materials and extraction liquid, affecting extraction efficiency, and long extraction time, achieving enhanced Skin stratum corneum barrier, improved mixing uniformity, and reduced extraction efficiency

Pending Publication Date: 2021-12-03
FROG PRINCE (FUJIAN) BABY & CHILD CARE PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The current extraction equipment has a relatively general effect on the mixing of materials and extracts. Usually, it only uses a single shaft-shaped part for mixing operations, and its efficiency is low;
[0006] (2) The current extraction equipment lacks the mixing effect in the vertical direction during the mixing process, resulting in insufficient contact between the material and the extraction liquid, resulting in a longer extraction time, which further affects its extraction efficiency

Method used

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  • Tianshan chlorella and Multi-stage cyclic extraction equipment and preparation method of chlorella deep purification factors
  • Tianshan chlorella and Multi-stage cyclic extraction equipment and preparation method of chlorella deep purification factors
  • Tianshan chlorella and Multi-stage cyclic extraction equipment and preparation method of chlorella deep purification factors

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Experimental program
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Effect test

Embodiment 1

[0047] Such as figure 1 As shown, the above-mentioned chlorella is subjected to wall-breaking treatment to obtain 50%-60% of chlorella wall-breaking algae powder. Specifically, the wall-breaking treatment uses a grinder to break the cells, and adds 6 times of water for external use. Circular extraction, the extraction temperature is 70°C, and the end point is determined according to the monitoring of the online spectrophotometer. The specific range is that the absorbance value at wavelength 240nm is diluted ≥0.9, and the absorbance at 550nm is ≤1.3. Release the extract to obtain A1; The glycerin solution is extracted by external circulation, the extraction temperature is 80°C, the extraction end point is 280nm absorbance value ten times dilution ≥ 1.2, 550nm absorbance ≤ 1.4, release the extract to obtain A2; add 2 times of 5% glycerin solution to algae residue Circular extraction, the extraction temperature is 80°C, the end point of the extraction is 280nm absorbance value 10...

Embodiment 2

[0052] Such as figure 1 As shown, the above-mentioned chlorella is subjected to wall-breaking treatment to obtain 50%-60% of chlorella wall-breaking algae powder. Specifically, a grinder machine is used for cell crushing, and 5 times of water is added for external circulation extraction, and the extraction The temperature is 80°C, and the end point is determined according to the online spectrophotometer monitoring. The specific range is that the absorbance value at 240nm is diluted ≥1.3, and the absorbance at 550nm≤1.6, and the extract is released to obtain A1; External circulation extraction, the extraction temperature is 70°C, the extraction end point is the absorbance value at 280nm, the tenfold dilution is ≥1.2, and the absorbance at 550nm is ≤1.3, and the extract is released to obtain A2; the dregs are added with 2 times of 5% glycerin solution for external circulation extraction. The temperature is 850°C, the end point of extraction is the absorbance value at 280nm, the ...

Embodiment 3

[0057] Such as figure 1 As shown, the above-mentioned chlorella is subjected to wall-breaking treatment to obtain 50%-60% of the chlorella wall-broken algae powder, and the cell is broken by a grinder machine, and 5 times of water is added for external circulation extraction, and the extraction temperature is 90°C , according to the online spectrophotometer monitoring to determine the end point, the specific range is that the absorbance value of wavelength 240nm is diluted by ten times ≥ 1.3, and the absorbance of 550nm is ≤ 1.5, and the extract is released to obtain A1; 4 times of 4% glycerin is added to the algae residue for external circulation extraction, The extraction temperature is 80°C, the end point of the extraction is the absorbance value at 280nm, the ten-fold dilution is ≥1.5, and the absorbance at 550nm is ≤1.4, and the extract is released to obtain A2; the algae residue is added with 3 times of 5% glycerin solution for external circulation extraction, and the ext...

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Abstract

The invention discloses Tianshan chlorella, which is named as Xinjiang Tianshan chlorella JY-QWWZ-1900386, is preserved in China General Microbiological Culture Collection Center (CGMCC) in June 2021, and has a preservation number of CGMCC NO:17926. The newly found Tianshan chlorella is subjected to enlarged culture through a bioreactor, and the Chlorella is extracted to prepare the chlorella deep purification factor. Newly developed multi-stage external circulation extraction equipment is used and comprises an extraction tank, a rotating shaft is rotationally mounted in the extraction tank, and a servo motor used for rotating the rotating shaft is arranged at the top of the extraction tank. According to the method, the compound extraction efficiency can be improved, the use of solvents and the dissolution of impurities can be reduced, the extraction end point is controlled by monitoring the dissolution of the effective components and the impurities in real time, the component stability of the product can be more accurately controlled, the effect stability of the product is further controlled, and the prepared chlorella deep purification factor is applied to the field of daily chemicals and has the effects of moisturizing, repairing and improving immunity.

Description

technical field [0001] The invention relates to the technical field of Tianshan chlorella and its extraction, in particular to the preparation method, application and multi-stage circulation extraction equipment of chlorella deep-layer purification factor, and belongs to the technical field of daily cosmetics. Background technique [0002] Chlorella deep purification factor is a mixture of various substances including proteins, polysaccharides, nucleic acids, peptides, vitamins and trace elements. Combining redox heavy metal ions, strengthening the stratum corneum barrier, regulating the immune function of macrophages in vitro, repairing cell and DNA damage, anti-pollution and anti-radiation, and it is extremely safe and non-irritating. [0003] Nowadays, the damage to children's skin due to environmental impact, living habits and bad cosmetics is becoming more and more serious, and the deep purification factor of chlorella has a good protective effect on human skin, especia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02A61K8/9722A61Q19/00B01F13/10B01F15/00B01F15/02
CPCB01D11/02A61K8/9722A61Q19/005A61K2800/805
Inventor 蒋旭峰李周欣李亮项光刚
Owner FROG PRINCE (FUJIAN) BABY & CHILD CARE PROD CO LTD
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