Polypeptide immune activator for improving insect resistance and disease resistance of rice

A technology of immune activation and disease resistance, applied in the field of rice pest control, to achieve the effect of improving tolerance and easy promotion

Pending Publication Date: 2021-12-07
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is worth noting that, unlike chewing insects, piercing-sucking brown planthoppers only cause small wounds on rice stems and leaves, and whether they can induce rice to produce Pep peptides has not y...

Method used

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  • Polypeptide immune activator for improving insect resistance and disease resistance of rice
  • Polypeptide immune activator for improving insect resistance and disease resistance of rice
  • Polypeptide immune activator for improving insect resistance and disease resistance of rice

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Experimental program
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Effect test

Embodiment 1

[0033] 1.1 Prediction of rice OsPROPEP gene by amino acid homologous sequence alignment

[0034] The present invention first utilizes the public database (NCBI: https: / / www.ncbi.nlm.nih.gov / ) to analyze the amino acid sequence of the immune small peptide Pep of multiple plant species ( figure 1). Through homologous comparison, it was found that 7 PROPEP homologous genes were found in the rice japonica (Oryzasativa.ssp.japonica) genome. The C-terminals of these OsPROPEPs have high similarity with other Pep small peptides of Poaceae, and they all have PGxPxEGxGGxGG conserved motifs. It is speculated that OsPep small peptides may be produced after the processing of these OsPROPEPs.

[0035] 1.2 Artificially synthesized OsPep small peptide

[0036] Many literatures have shown that exogenous application of artificially synthesized Arabidopsis thaliana AtPep small peptides and ZmPep small peptides can activate the immune response of plant cells. According to the predicted OsPep ...

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PUM

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Abstract

The invention discloses a polypeptide immune activator for improving insect resistance and disease resistance of rice. The polypeptide composition contains any one of the following polypeptides as an active ingredient: OsPep1: ARLRPKPPGNPREGSGGNGGHHH; OsPep2: DDSKPTRPGAPAEGSGGNGGAIH; OsPep3: ADSAPQRPGSPAEGAGGNGGAVH; and OsPep7: SKPKPEPPGYPREGGGGNGGVVD. According to the activator, by externally spraying the polypeptide immune activator, the natural immune defense mechanism of the rice is activated, insect-resistant and disease-resistant secondary metabolites are synthesized, and the tolerance of the rice to plant diseases and insect pests is improved. Compared with traditional pesticides, the polypeptide immune activator is non-toxic, pollution-free, easy to degrade and free of residues; and compared with cultivation of disease-resistant and insect-resistant rice varieties, the polypeptide immune activator is efficient, convenient and easy to popularize.

Description

Technical field: [0001] The invention belongs to the field of rice pest control, and in particular relates to a polypeptide immune activator for improving rice resistance to insects and diseases. Background technique: [0002] Rice is the main food crop related to the national economy and people's livelihood. Brown planthopper (Brown planthopper, Nilaparvatalugens ) is a migratory pest that sucks rice specially, which seriously threatens the safe production of rice in my country. South China, Jiangnan, and the middle and lower reaches of the Yangtze River are the areas most severely damaged by rice planthoppers, and the occurrence area of ​​rice planthoppers ranks first among rice pests and diseases. Rice blast is a fungal disease caused by the infection of Magnaporthe oryzae. It is one of the most serious diseases that harm rice production in my country. When it occurs, it will cause serious grain production reduction. cancer". At present, the application of chemical pe...

Claims

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Application Information

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IPC IPC(8): C07K14/415A01G22/22A01G13/00A01G7/06
CPCC07K14/415A01G22/22A01G13/00A01G7/06
Inventor 李剑峰沈文忠
Owner SUN YAT SEN UNIV
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