Traditional Chinese medicine residue fermentation compound and preparation method and application thereof
A technology of traditional Chinese medicine dregs and compounds, which is applied in the fields of application, animal feed, animal feed, etc., can solve the problems of environmental pollution, waste of resources, etc., and achieve the effect of simple preparation method, cost saving, and great promotion and application prospects
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[0024] In a second aspect, the present invention provides a method for preparing a fermentation compound of traditional Chinese medicine dregs, which comprises the following steps:
[0025] Step 1, Chinese medicinal dregs are fermented for the first time with Inonotus obliquus to obtain a pretreatment liquid of traditional Chinese medicinal dregs.
[0026] In the present invention, Inonotus obliquus, preferably Inonotus obliquus BNCC117822, can be purchased from the market, for example, from Beijing Beina Chuanglian Biotechnology Research Institute.
[0027] In a preferred embodiment of the present invention, in order to make the traditional Chinese medicine dregs ferment evenly and completely, it also includes pretreatment of the traditional Chinese medicine dregs before step 1, the pretreatment includes washing, drying, grinding, sieving, and collecting 40~ 140 purpose traditional Chinese medicine dregs. Specifically, wash the dregs of traditional Chinese medicine with dist...
Embodiment 1
[0090] (1) Pretreatment of traditional Chinese medicine dregs:
[0091] Wash 3 times with distilled water, dry, grind and sieve under natural conditions, collect 60-120 mesh dregs particles, pack Store in a dry place after bagging.
[0092] (2) Preparation of medium:
[0093] Agar medium (w / v): 0.3g peptone, 1.5g agar, 3g malt powder, keep the pH natural;
[0094] Liquid medium (w / v): CaCl 2 0.01g, glucose 2g, yeast extract 0.1g, KH 2 PO 4 0.1g, peptone 0.3g, MgSO 4 0.15g, dilute to 100mL with distilled water, keep the pH natural;
[0095] Fermentation medium (w / v): corn flour (hydrolyzed) 3.0g, (NH 4 ) 2 SO 4 0.222g, CoCl 2 ·6H 2 O 0.002g, FeSO 4 ·7H 2 O 0.005g, KH 2 PO 4 0.004g, MgSO 4 ·7H 2 O 0.02g, ZnSO 4 ·7H 2 O 0.001g, MnCl 2 4H 2 O0.009g, CaCl 2 0.05g, Tween 800.43mL, dilute to 100mL with distilled water, adjust the pH value to 4.3.
[0096] (3) Preparation process of traditional Chinese medicine dregs fermentation compound
[0097] Inonotus obli...
Embodiment 2~5
[0103] The process is similar to Example 1, the only difference is that the nitrogen source in the fermentation medium is different, and the nitrogen source is beef extract, peptone, yeast extract or urea in sequence.
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